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首页> 外文期刊>Integrative Biology: quantitative biosciences from nano to macro >A stretching device for imaging real-time molecular dynamics of live cells adhering to elastic membranes on inverted microscopes during the entire process of the stretch
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A stretching device for imaging real-time molecular dynamics of live cells adhering to elastic membranes on inverted microscopes during the entire process of the stretch

机译：拉伸装置，用于在拉伸的整个过程中对附着在倒置显微镜上的弹性膜上的活细胞进行实时分子动力学成像

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摘要

We present a device for stretching cells adhering to elastic membranes in equiaxial or uniaxial mode, meanwhile allowing real-time imaging of molecular dynamics of live cells at high resolution on an inverted microscope during the entire process of the stretch. We obtained high-resolution images of stress fibers at each stage of the stretch, and found that stress fibers were shortened after one stretching cycle. We, for the first time, captured real-time images of the process of stress fiber disassembly during stretching. Several adjacent stress fibers appeared to reassemble into a single one after stretching. All these indicated that mechanical stretching played important roles in the rearrangement of actin. laments. This device will be especially useful in studies of the molecular dynamics in the process of mechanotransduction. The device is fabricated on a glass slide through a simple procedure and is adaptable to most ordinary laboratories.
机译：我们提出了一种用于以等轴或单轴模式拉伸粘附在弹性膜上的细胞的设备，同时允许在拉伸的整个过程中以高分辨率在倒置显微镜上实时成像活细胞的分子动力学。我们在拉伸的每个阶段获得了应力纤维的高分辨率图像，发现应力纤维在一个拉伸周期后会缩短。我们首次捕获了拉伸过程中应力纤维拆卸过程的实时图像。拉伸后，几根相邻的应力纤维似乎重新组装成一条。所有这些表明机械拉伸在肌动蛋白的重排中起重要作用。感叹。该装置在机械转导过程中的分子动力学研究中将特别有用。该设备可以通过简单的步骤在载玻片上制造，并且适用于大多数普通实验室。

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