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The regeneration and augmentation of bone with injectable osteogenic cell sheet in a rat critical fracture healing model

机译：可注射成骨细胞片在大鼠严重骨折愈合模型中的骨再生和增生

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Limitations in the current treatment strategies make cases with compromised bone healing challenging clinical problems. Osteogenic cell sheets (OCSs), fabricated from rat bone marrow stromal cells (BMSCs), contain enriched osteoblasts and extracellular matrix. Here, we evaluated whether the minimally invasive percutaneous injection of OCSs without a scaffold could be used as a treatment to increase bone regeneration in a critical fracture healing model. Critical fracture healing model was created in the femora of 60 male Fischer 344 inbred rats using marrow ablation and periosteal removal. The rats were then randomly divided into two groups. Six hours after fracture, one group received an injection of OCSs (OCS group), while the second group was injected with phosphate-buffered saline (PBS) (control group). Fracture healing was evaluated using radiological, histological, micro-computed tomography (CT) and biomechanical analyses. The radiological and histological evaluations demonstrated enhanced bone regeneration in the OCS group compared with that in the control group. By 12 weeks, the hard callus had been remodelled via recorticalization in the OCS group. By contrast, no fracture union was found in the rats in the control group. Biomechanical testing revealed a significantly higher maximum bending load in the OCS group compared with that in the control group. The results of the present study demonstrate that the injection of entire OCSs can enhance bone regeneration and lead to bony union in a critical fracture healing model. Therefore, this procedure offers a minimally invasive technique to promote hard tissue reconstruction and, in particular, bone repair strategies for cases with compromised bone healing. (C) 2015 Elsevier Ltd. All rights reserved.
机译：当前治疗策略的局限性使骨愈合不良的病例成为临床难题。由大鼠骨髓基质细胞（BMSC）制成的成骨细胞片（OCS）含有丰富的成骨细胞和细胞外基质。在这里，我们评估了无支架的OCS的微创经皮注射是否可以用作增加关键骨折愈合模型中骨再生的治疗方法。使用骨髓消融和骨膜切除术在60只雄性Fischer 344自交系大鼠的股骨中创建临界骨折愈合模型。然后将大鼠随机分为两组。骨折后六小时，一组接受OCS注射（OCS组），而第二组注射磷酸盐缓冲盐水（PBS）（对照组）。使用放射学，组织学，微型计算机断层扫描（CT）和生物力学分析评估骨折愈合。放射学和组织学评估显示，与对照组相比，OCS组的骨再生增强。到12周时，OCS组中的硬皮层已通过皮层修复进行了重塑。相比之下，对照组中未发现骨折愈合。生物力学测试表明，OCS组的最大弯曲负荷明显高于对照组。本研究的结果表明，在关键的骨折愈合模型中，注射整个OCS可以增强骨骼再生并导致骨结合。因此，该程序提供了一种微创技术来促进硬组织的重建，尤其是对于骨愈合不良的病例的骨修复策略。 （C）2015 Elsevier Ltd.保留所有权利。

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