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首页> 外文期刊>Injury >The epitope characterisation and the osteogenic differentiation potential of human fat pad-derived stem cells is maintained with ageing in later life.
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The epitope characterisation and the osteogenic differentiation potential of human fat pad-derived stem cells is maintained with ageing in later life.

机译：人脂肪垫衍生干细胞的表位表征和成骨分化潜能随着衰老而保持。

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Some clinical settings are deficient in osteogenic progenitors, e.g. atrophic nonunited fractures, large bone defects, and regions of scarring and osteonecrosis. These benefit from the additional use of bone marrow-derived mesenchymal stem cells, but these cells exhibit an age-related decline in lifespan, proliferation and osteogenic potential. Therapeutic approaches for the repair of bone could be optimised by the identification of a stem cell source that does not show age-related changes. Fat pad-derived stem cells are capable of osteogenesis, but a detailed study of the effect of ageing on their epitope profile and osteogenic potential has so far not been performed. Fat pad-derived cells were isolated from 2 groups of 5 patients with a mean age of 57 years (S.D. 3 years) and 86 years (S.D. 3 years). The proliferation, epitope profile and osteogenic differentiation potential of cells from the 2 groups were compared. Cells isolated from the fat pad of both groups showed similar proliferation rates andexhibited a cell surface epitope profile similar but not identical to that of bone marrow-derived stem cells. The cells from both groups cultured in osteogenic medium exhibited osteogenesis as shown by a significant upregulation of alkaline phosphatase and osteocalcin genes, and significantly greater alkaline phosphatase enzyme activity compared to cells cultured in the control medium. The cells cultured in the osteogenic medium also showed greater calcium phosphate deposition on alizarin red staining. There was no significant difference between the osteogenic potential of the two age groups for any of the parameters studied. The fat pad is a consistent and homogenous source of stem cells that exhibits osteogenic differentiation potential with no evidence of any decline with ageing in later life. This has many potential therapeutic tissue engineering applications for the repair of bone defects in an increasingly ageing population.
机译：一些临床环境缺乏成骨祖细胞，例如萎缩性不合并骨折，大骨缺损以及疤痕和骨坏死区域。这些受益于骨髓来源的间充质干细胞的额外使用，但是这些细胞在寿命，增殖和成骨潜能方面表现出与年龄相关的下降。可以通过鉴定不显示与年龄相关的变化的干细胞来源来优化修复骨的治疗方法。源自脂肪垫的干细胞具有成骨能力，但是迄今为止，尚未进行衰老对其表位特征和成骨潜能影响的详细研究。从2名5位患者的组中分离出脂肪垫衍生的细胞，平均年龄为57岁（标准年3岁）和86岁（标准年3岁）。比较了两组细胞的增殖，表位特征和成骨分化潜能。从两组的脂肪垫分离的细胞表现出相似的增殖速率，并且显示出与骨髓干细胞相似但不相同的细胞表面表位特征。与在对照培养基中培养的细胞相比，在成骨培养基中培养的两组细胞均表现出成骨性，如碱性磷酸酶和骨钙素基因的显着上调以及碱性磷酸酶的活性明显更高。在成骨培养基中培养的细胞在茜素红染色上也显示出更大的磷酸钙沉积。对于所研究的任何参数，两个年龄组的成骨潜能之间无显着差异。脂肪垫是干细胞的一致且均质的来源，具有成骨分化潜能，没有证据表明其寿命会随着衰老而下降。这对于修复日益老龄化的人群中的骨缺损具有许多潜在的治疗性组织工程应用。

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