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首页> 外文期刊>Brain research. Brain research protocols >A novel method of detecting rCBF with laser-Doppler flowmetry without cranial window through the skull for a MCAO rat model.
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A novel method of detecting rCBF with laser-Doppler flowmetry without cranial window through the skull for a MCAO rat model.

机译：一种用于MCAO大鼠模型的无颅窗的激光多普勒血流仪检测rCBF的新方法。

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BACKGROUND AND PURPOSE: In a rat model of middle cerebral artery occlusion (MCAO) with intraluminal technique, lesion volume and its reproducibility vary among laboratories. Although laser-Doppler flowmetry (LDF) is useful to optimize the reliability, conventional methods require a craniotomy and special apparatus. The purpose of this study was to evaluate a novel approach for LDF monitoring of rCBF through lateral aspect of the skull without a craniotomy. METHODS: SD rats were subjected to 45 min of MCAO using an intraluminal thread. MCAO was achieved by an examiner who had been trained 4 weeks for making the model with no LDF monitoring (Group-1, n = 12), while in the other group, the same examiner induced MCAO using a novel approach of LDF monitoring (Group-2, n = 12). rCBF was detected through an LDF probe attached to the lateral aspect of the skull. The survival rate and the infarct volume were estimated for comparison between the two groups 2 days after MCAO. RESULTS: The mortality rate was 25% in Group-1 and 0% in Group-2. The lesion volume of the cortex in Group-2 was 167.21 +/- 48.54 mm(3) (mean +/- SD), which was larger than that in Group-1 (112.77 +/- 36.03 mm(3), P = 0.026). The coefficient variation of the lesion volume was smaller in Group-2 (29%) than in Group-1 (35%), indicating better reproducibility of the lesion volume in Group-2 than in Group-1. CONCLUSIONS: The approach of LDF monitoring through the lateral aspect of skull was useful for making large consistent infarct with reducing intraanimal variability and unexpected animal death for rat MCAO model.
机译：背景与目的：在腔内技术大鼠大脑中动脉闭塞（MCAO）的模型中，病灶体积及其可重复性在实验室之间存在差异。尽管激光多普勒血流仪（LDF）可用于优化可靠性，但传统方法需要开颅手术和专用设备。这项研究的目的是评估一种无需颅骨切开术即可通过颅骨侧面对LCBF进行LDF监测的新方法。方法：SD大鼠使用腔内线进行MCAO 45分钟。 MCAO由一名经过4周训练，没有模型进行LDF监测的模型的培训者完成（第1组，n = 12），而在另一组中，同一研究者使用新的LDF监测方法诱导了MCAO（组） -2，n = 12）。通过连接至颅骨外侧的LDF探针检测到rCBF。估计MCAO后2天的两组的存活率和梗塞体积以进行比较。结果：第一组的死亡率为25％，第二组的死亡率为0％。第2组中皮层的病变体积为167.21 +/- 48.54 mm（3）（平均+/- SD），大于第1组中的皮损（112.77 +/- 36.03 mm（3），P = 0.026）。组2中病变体积的系数变化（29％）小于组1中病变的系数变化（35％），表明组2中病变体积的再现性高于组1。结论：通过颅骨侧面进行LDF监测的方法对于减少MCAO模型的动物内部变异性和减少动物意外死亡，有助于形成较大的持续性梗塞。

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