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首页> 外文期刊>Brain research. Brain research protocols >The isolated perfused bovine retina--a sensitive tool for pharmacological research on retinal function.
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The isolated perfused bovine retina--a sensitive tool for pharmacological research on retinal function.

机译：离体的灌注牛视网膜-一种用于视网膜功能药理研究的敏感工具。

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The electroretinogram (ERG) of the isolated bovine retina serves as a proven criterion of retinal activity. It is used as a sensitive pharmacological tool for testing effects of applied drugs and toxins on photoreceptors, and higher order neurons that contribute to the generation of the b-wave. Following isolation and detachment from the underlying pigment epithelium, part of the retina was mounted into a closed chamber and perfused by a nutrient solution. Flow rate of the nutrient solution and its ingredients, incubation temperature and light intensity were optimised empirically to achieve a maximum b-wave amplitude. Under these conditions, a reproducible, high-resolution ERG can be stably recorded for more than 10 h with sufficient oxygenation found to be a prerequisite for the long-lasting stability. Addition of L(+)glutamate to the nutrient solutions was not anymore beneficial for the b-wave amplitude. A well-known inhibitor of oxidative phosphorylation (KCN) and antagonists of voltage-gated Ca2+ channels (isradipine, omega-conotoxin-GVIA and NiCl2) were used to prove the validity of the test system. The recording of the ERG from the isolated and perfused bovine retina serves as a valuable physiological model for a neuronal network in which important questions related to the retinal signalling and metabolism can be investigated.
机译：隔离的牛视网膜的视网膜电图（ERG）可作为视网膜活性的可靠标准。它被用作敏感的药理学工具，用于测试所用药物和毒素对光感受器和有助于产生b波的高级神经元的作用。从下面的色素上皮中分离和脱离后，将视网膜的一部分安装到密闭腔室中，并用营养液灌注。营养液及其成分的流速，孵育温度和光强度均根据经验进行了优化，以实现最大的b波振幅。在这些条件下，可稳定记录可再现的高分辨率ERG超过10小时，而充分的充氧被认为是持久稳定的先决条件。向营养液中添加L（+）谷氨酸不再对b波振幅有益。众所周知的氧化磷酸化抑制剂（KCN）和电压门控的Ca2 +通道（伊斯拉平，ω-芋螺毒素GVIA和NiCl2）的拮抗剂被用来证明测试系统的有效性。从分离和灌注的牛视网膜中记录ERG可以作为神经元网络的有价值的生理模型，在其中可以研究与视网膜信号传导和代谢有关的重要问题。

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