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首页> 外文期刊>International Biodeterioration & Biodegradation >Cyanobacterial diversity in hydrocarbon-polluted sediments and their possible role in bioremediation
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Cyanobacterial diversity in hydrocarbon-polluted sediments and their possible role in bioremediation

机译：碳氢化合物污染沉积物中的蓝细菌多样性及其在生物修复中的可能作用

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摘要

The cyanobacterial diversity in crude-oil-contaminated mats and soil sediments from the Guwahati refinery, in Guwahati, India, was studied using a polyphasic approach. Sequencing of the 16S rRNA gene of the isolated cyanobacterial strains and of denaturing gradient gel electrophoresis bands of the environmental samples revealed dominance of the genera Leptolyngbya and Planktothrix and the presence of Tolypothrix sp., Anabaena sp., Oscillatoriales sp., and Arthronema sp. These strains exhibited wide variation in their transferase glutamine synthetase activity and ammonia excretion activity. Treatment of isolates with hydrocarbon sludge caused a sharp decrease in photosynthetic oxygen evolution activity followed by time-dependent recovery, which ranged from 18.6% to 153.1% after 4 h. The CO2 evolution activity remained unaffected or exhibited stimulation after 1% or 2% oil sludge treatment. (C) 2015 Elsevier Ltd. All rights reserved.
机译：使用多相方法研究了印度古瓦哈提古瓦哈提炼油厂的原油污染垫层和土壤沉积物中的蓝细菌多样性。分离的蓝细菌菌株的16S rRNA基因的测序和环境样品的变性梯度凝胶电泳带显示了Leptolyngbya和Planktothrix属的优势，以及Tolypothrix sp。，Anabaena sp。，Oscillatoriales sp。和Arthronema sp。的存在。这些菌株在其转移酶谷氨酰胺合成酶活性和氨排泄活性方面表现出很大的差异。用碳氢化合物污泥处理分离株会导致光合作用的放氧活性急剧下降，然后随时间而恢复，4小时后恢复率为18.6％至153.1％。 1％或2％的油泥处理后，CO2释放活性保持不变或受到刺激。 （C）2015 Elsevier Ltd.保留所有权利。

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