...
首页> 外文期刊>International Biodeterioration & Biodegradation >On the bacterial communities associated with the corrosion product layer during the early stages of marine corrosion of carbon steel
【24h】

On the bacterial communities associated with the corrosion product layer during the early stages of marine corrosion of carbon steel

机译:碳钢海洋腐蚀早期与腐蚀产物层相关的细菌群落

获取原文
获取原文并翻译 | 示例
           

摘要

Carbon steel coupons were immersed for 1-8 weeks at similar to 1 m deep in a French harbor of the Atlantic coast. The resulting corrosion product layers were characterized by mu-Raman spectroscopy. They consisted of an inner stratum of sulfated green rust covered by an outer stratum of lepidocrocite (predominantly), goethite and magnetite. Mackinawite FeS was detected after one month of immersion, but only locally. The bacterial characterization of these layers was coupled to this analysis. The diversity of the culturable bacteria grown in liquid media directly from biofilms or isolated on agar plates was evaluated by 165 rRNA gene sequencing. The microbial diversity was also estimated, without cultivation and after Temporal Temperature Gradient Electrophoresis (TTGE) separation. A moderate biodiversity was found in all cases and different bacteria associated with the redox cycles of Fe and S were identified. Roseobacter, Erythrobacter and Bacillus were dominant among culturable bacteria whereas Sulfurimonas autotrophica, a sulfur-oxidizing bacterium, was detected among the total bacterial community at all immersion times. After one month of immersion, sulfide-producing bacteria were detected, e.g. Desulfovibrio profundus in liquid mixed cultures and Sulfurospirillum arcachonense in the total bacterial community, in agreement with the local identification of FeS by mu-Raman spectroscopy. (C) 2015 Elsevier Ltd. All rights reserved.
机译:将碳钢试样在大西洋沿岸的法国海港中浸入约1 m深,浸泡1-8周。通过mu-拉曼光谱法表征所得的腐蚀产物层。它们由硫酸化的绿色锈的内部层组成,上面覆盖着纤铁矿(主要是针铁矿和磁铁矿)的外部层。浸入水中一个月后检测到了Mackinawite FeS,但仅限于局部。这些层的细菌特征与该分析相结合。直接通过生物膜在液体培养基中生长或在琼脂板上分离的可培养细菌的多样性通过165 rRNA基因测序进行了评估。还估计了微生物的多样性,无需培养,并且经过温度梯度电泳(TTGE)分离。在所有情况下均发现了适度的生物多样性,并鉴定了与Fe和S的氧化还原循环有关的不同细菌。在所有可浸入细菌中,罗斯福细菌,红细菌和芽孢杆菌在可培养细菌中占主导地位,而总硫菌中检测到自养硫脲菌(Sulfurimonas autotrophica)是一种硫氧化细菌。浸泡一个月后,检测到产生硫化物的细菌,例如混合培养液中的深层脱硫弧菌和总细菌群落中的弓形硫螺旋藻,与通过拉曼光谱法对FeS的局部鉴定相一致。 (C)2015 Elsevier Ltd.保留所有权利。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号