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首页> 外文期刊>International Biodeterioration & Biodegradation >Biotransformation of tributyltin chloride to less toxic dibutyltin dichloride and monobutyltin trichloride by Klebsiella pneumoniae strain SD9
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Biotransformation of tributyltin chloride to less toxic dibutyltin dichloride and monobutyltin trichloride by Klebsiella pneumoniae strain SD9

机译：肺炎克雷伯氏菌SD9将三丁基氯化锡生物转化为毒性较小的二丁基二氯化锡和三丁基单丁基锡

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摘要

A tributyltin chloride (TBTCl) resistant bacterial strain was isolated from the Zuari estuary, in Goa, India, and identified as Klebsiella pneumoniae based on biochemical characteristics and 16S rRNA sequence analysis, and designated as strain SD9. It could utilize TBTCl as a sole carbon source in mineral salt medium and tolerated up to 2.5 mM TBTCl with maximum growth at 2 mM. Nuclear magnetic resonance spectroscopic analysis of column purified TBTCl degradation products clearly demonstrated the presence of dibutyltin dichloride (DBTCl2) and monobutyltin trichloride (MBTCl3). Mass spectrometry further confirmed degradation of toxic TBTCl into its less toxic derivatives, viz., DBTCl2 and MBTCl3. This strain also showed enhanced siderophore production in the presence of TBTCl, which was demonstrated by chrome azurol S agar assay as an increase in diameter of the orange halo around the bacterial colony in the presence of 2 mM TBTCl; this seems to be a mechanism to counteract TBTCl toxicity. Furthermore, scanning electron microscopy revealed significant morphological alterations as shrinkage in cell size along with roughness of cell surface when bacterial cells were exposed to 2 mM TBTCl. These interesting characteristics of this estuarine bacterium make it a potential tool for bioremediation of TBTCl-contaminated sites since it possesses biotransformation capability to convert TBICl into DBTCl2 and MBTCl3. (c) 2015 Elsevier Ltd. All rights reserved.
机译：从印度果阿的祖阿里河口分离出对三丁基氯化锡（TBTC1）具有抗性的细菌菌株，并根据生化特征和16S rRNA序列分析将其鉴定为肺炎克雷伯菌，并命名为SD9菌株。它可以利用TBTCl作为矿物盐介质中的唯一碳源，并耐受高达2.5 mM的TBTCl，最大生长量为2 mM。柱纯化的TBTC1降解产物的核磁共振波谱分析清楚地表明了二氯化二丁基锡（DBTC12）和三氯化单丁基锡（MBTC13）的存在。质谱进一步证实了毒性TBTC1的降解为其毒性较低的衍生物，即DBTCl2和MBTCl3。在TBTC1存在下，该菌株还显示出增加的铁载体生成，这通过铬天青S琼脂分析证实，在存在2mM TBTCl的情况下，细菌菌落周围的橙色光晕直径增加。这似乎是抵消TBTC1毒性的机制。此外，当细菌细胞暴露于2mM TBTCl时，扫描电子显微镜揭示了显着的形态学改变，如细胞大小的收缩以及细胞表面的粗糙度。该河口细菌的这些有趣特征使其成为生物修复TBTCl污染位点的潜在工具，因为它具有将TBICl转化为DBTCl2和MBTCl3的生物转化能力。 （c）2015 Elsevier Ltd.保留所有权利。

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