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首页> 外文期刊>International immunopharmacology >Renoprotective mechanisms of morin in cisplatin-induced kidney injury
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Renoprotective mechanisms of morin in cisplatin-induced kidney injury

机译：茉莉酸在顺铂引起的肾脏损伤中的肾脏保护机制

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In this study, we investigated the renoprotective effects of morin on cisplatin-induced kidney injury in mice. Serum creatinine and blood urea nitrogen. (BUN) levels, glutathione peroxidase (GSH-PX) and superoxide dismutase (SOD) activities were determined according to the corresponding kits. The mRNA levels of TNF-alpha and IL-1 beta in kidney tissues were measured by quantitative real-time PCR (qRT-PCR). The activities of cytochrome P450 2E1 (CYP2E1), nuclear factor kappa B (NF-kappa B) p65, P38 mitogen-activated protein kinase (MAPK), Bax, p53 and cleaved caspase 3 were evaluated by western blotting. The results showed that the model of cisplatininduced kidney injury was successfully replicated, and morin significantly attenuated histopathological changes and decreased the levels of TNF-alpha and IL-1 beta in the kidneys. In addition, morin attenuated the activation of CYP2E1, phospho-NF-kappa B p65, phospho-P38 MAPK, Bax, phospho-p53 and cleaved caspase 3 in CP-induced kidney injury. In conclusion, these results indicated that the renoprotective mechanisms of morin may be attributed to the suppression of oxidative stress, inflammation and apoptosis in CP-induced kidney injury. (C) 2015 Elsevier B.V. All rights reserved.
机译：在这项研究中，我们调查了茉莉酸对顺铂诱导的小鼠肾脏损伤的肾脏保护作用。血清肌酐和血尿素氮。 （BUN）水平，谷胱甘肽过氧化物酶（GSH-PX）和超氧化物歧化酶（SOD）活性根据相应的试剂盒测定。通过定量实时PCR（qRT-PCR）测量肾脏组织中TNF-α和IL-1β的mRNA水平。通过蛋白质印迹法评估细胞色素P450 2E1（CYP2E1），核因子κB（NF-κB）p65，P38丝裂原活化蛋白激酶（MAPK），Bax，p53和裂解的胱天蛋白酶3的活性。结果表明，成功地复制了顺铂引起的肾脏损伤模型，morin明显减轻了肾脏的组织病理学变化，并降低了TNF-α和IL-1β的水平。此外，morin减弱了CP诱导的肾损伤中CYP2E1，磷酸化NF-κBp65，磷酸化-P38 MAPK，Bax，磷酸化-p53和裂解的胱天蛋白酶3的激活。总之，这些结果表明，morin的肾脏保护机制可能归因于CP诱导的肾损伤中氧化应激，炎症和细胞凋亡的抑制。 （C）2015 Elsevier B.V.保留所有权利。

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