Paeoniflorin (PF), one of the major active ingredients of Chinese peony, has demonstrated anti-inflammatory and immunoregulatory effects. However, it has remained unclear whether PF treatment can inhibit allergic inflammation in asthma. In this study, we evaluated the effects of PF on pulmonary function and airway inflammation in asthmatic mice. The allergic asthma models were established in BALB/c mice. The mice were sensitized and challenged with ovalbumin. Airway hyperresponsiveness was detected by direct airway resistance analysis. Lung tissues were examined for inflammatory cell infiltration. IL-5, IL-13, IL-17, and eotaxin in bronchoalveolar lavage fluid (BALF) and their mRNA expression in lung tissue were examined by ELISA and realtime PCR, respectively. The total IgE level in serum was measured by ELISA. The protein expression of p-ERK and p-JNK was detected by western blot. Our data showed that PF oral administration significantly reduced airway hyperresponsiveness to aerosolized methacholine and decreased IL-5, IL-13, IL-17 and eotaxin levels in the BALF, and decreased IgE level in the serum. Histological studies showed that PF administration markedly decreased inflammatory infiltration. Similarly, treatment with PF significantly inhibited IL-5, IL-13, IL-17 and eotaxin mRNA expression in lung tissues. The protein expression levels of p-ERK and p-JNK were substantially decreased after oral administration of PF. In summary, PF displayed anti-inflammatory effects in the OVA-induced asthmatic model by decreasing the expression of IL-5, IL-13, IL-17 and eotaxin. These effects were mediated at least partially by inhibiting the activation of MAPK pathway. (C) 2014 Elsevier B.V. All rights reserved.
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机译：eon药苷（PF）是中国牡丹的主要活性成分之一，具有抗炎和免疫调节作用。但是，尚不清楚PF治疗能否抑制哮喘的过敏性炎症。在这项研究中，我们评估了PF对哮喘小鼠肺功能和气道炎症的影响。在BALB / c小鼠中建立了过敏性哮喘模型。使小鼠敏化并用卵清蛋白攻击。通过直接气道阻力分析检测气道高反应性。检查肺组织的炎性细胞浸润。分别通过ELISA和实时PCR检测支气管肺泡灌洗液（BALF）中的IL-5，IL-13，IL-17和嗜酸性粒细胞趋化因子及其在肺组织中的mRNA表达。通过ELISA测量血清中的总IgE水平。 Western blot检测p-ERK和p-JNK的蛋白表达。我们的数据显示，PF口服给药可显着降低气雾剂对气雾化乙酰甲胆碱的过敏反应，并降低BALF中的IL-5，IL-13，IL-17和嗜酸性粒细胞趋化因子水平，并降低血清中的IgE水平。组织学研究表明，PF的使用显着减少了炎症浸润。同样，用PF处理可显着抑制肺组织中IL-5，IL-13，IL-17和嗜酸性粒细胞趋化因子mRNA的表达。口服PF后，p-ERK和p-JNK的蛋白表达水平明显降低。总之，PF通过降低IL-5，IL-13，IL-17和嗜酸性粒细胞趋化因子的表达在OVA诱发的哮喘模型中表现出抗炎作用。这些作用至少部分地通过抑制MAPK途径的激活而介导。 （C）2014 Elsevier B.V.保留所有权利。
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