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首页> 外文期刊>International Dairy Journal >Performance of spontaneous phage-resistant derivatives of Lactobacillus plantarum in fermented milk manufacture.
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Performance of spontaneous phage-resistant derivatives of Lactobacillus plantarum in fermented milk manufacture.

机译：植物乳杆菌自发抗噬菌体衍生物在发酵乳生产中的性能。

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A total of 81 spontaneous phage-resistant mutants were isolated from two sensitive Lactobacillus plantarum strains (ATCC 8014 and PLN) using two lytic bacteriophages (ATCC 8014-B1 and ATCC 8014-B2), either separately or mixed in a phage cocktail. Phenotypic characteristics related to their phage resistance abilities, as well as their technological properties, were determined. Random amplification of polymorphic DNA (RAPD-PCR) was used to determine genetic diversity among the selected isolates and their respective parent strains. Most of the mutants isolated were completely or partially unable to adsorb phage particles. Stability, high level of resistance and adequate technological properties were exhibited by the isolates of Lb. plantarum ATCC 8014. A selected variant, MC4, was able to resist the mixture of both lytic phages during fermented milk manufacture and the subsequent refrigerated storage. This phage-resistant derivative might be used in culture rotation programs when commercial strains become sensitive to phages present in industrial environments
机译：使用两个裂解噬菌体（ATCC 8014-B1和ATCC 8014-B2）分别从两个敏感的植物乳杆菌菌株（ATCC 8014和PLN）中分离出总共81个自发噬菌体抗性突变体在噬菌体鸡尾酒中。确定了与它们的噬菌体抗性能力有关的表型特征，以及它们的技术特性。多态性DNA的随机扩增（RAPD-PCR）用于确定所选菌株及其各自亲本菌株之间的遗传多样性。分离出的大多数突变体完全或部分不能吸附噬菌体颗粒。 Lb的分离物表现出稳定性，高水平的抗性和足够的技术性能。植物乳杆菌ATCC8014。选择的变体MC4能够在发酵乳​​制造和随后的冷藏中抵抗两种裂解噬菌体的混合物。当商业菌株对工业环境中存在的噬菌体敏感时，这种抗噬菌体的衍生物可用于培养轮换程序

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