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首页> 外文期刊>International journal of antimicrobial agents >Prevalence of extended spectrum beta-lactamases among Enterobacteriaceae: an Italian survey
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Prevalence of extended spectrum beta-lactamases among Enterobacteriaceae: an Italian survey

机译：肠杆菌科中广谱β-内酰胺酶的流行：意大利调查

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The prevalence of extended-spectrum beta-lactamase (ESBL) production by consecutive non-repeated isolates of Enterobacteriaceae was determined over a 6-month period. A total of 8015 strains were isolated from ten Italian laboratories and 509 (6.3%) of these were designated ESBL producers from the results of a double-disk synergy test. Eseherichia coli was the most isolated microrganism, followed by Klebsiella pneumoniae and Proteus mirabilis. Providencia stuartii (28.1%) was the most frequently isolated ESBL producer, followed by K. pneumoniae and Enterobacter aerogenes (20.5%). However, amongst all ESBL producers, K. pneumoniae (38.2%) was the most represented followed by P. mirabilis (25.7%). All the strains positive to DID tests were confirmed for the carriage of TEM and SHV genes using colony-blot hybridisation (CH). A total of 447 strains (88.0%) were CH-positive, of which 42.3% hybridised with the TEM-type probe, 30.1% with the SHV-type probe and 15.6% with both probes. In conclusion, our findings indicate that 6.3% of all Enterobacteriaceae tested produced ESBLs, 42.3% of which were TEM-derived enzymes. More than 20% of P. stuartli, K. pneumoniae and E. aerogenes harbour these enzymes. The double-disk test seems to be a useful test to identify ESBL producing strains. (C) 2002 Elsevier Science B.V. and International Society of Chemotherapy. All rights reserved. [References: 27]
机译：在六个月的时间内确定了肠杆菌科连续非重复分离株产生的广谱β-内酰胺酶（ESBL）的发生率。从双盘协同试验的结果中，从十个意大利实验室中分离出8015株菌株，其中509（6.3％）被指定为ESBL生产者。大肠杆菌是最孤立的微生物，其次是肺炎克雷伯菌和奇异变形杆菌。斯图亚特氏普罗旺斯菌（28.1％）是最常分离的ESBL生产者，其次是肺炎克雷伯菌和产气肠杆菌（20.5％）。但是，在所有ESBL生产者中，肺炎克雷伯菌（38.2％）的比例最高，其次是奇异假单胞菌（25.7％）。使用菌落印迹杂交（CH）确认所有对DID测试呈阳性的菌株均携带TEM和SHV基因。共有447株菌株（88.0％）为CH阳性，其中42.3％与TEM型探针杂交，30.1％与SHV型探针杂交，两种探针均为15.6％。总之，我们的发现表明，在所有测试的肠杆菌科中，有6.3％产生了ESBL，其中42.3％是TEM衍生的酶。超过20％的斯图亚特氏菌，肺炎克雷伯菌和产气链球菌都含有这些酶。双盘测试似乎是鉴定ESBL产生菌株的有用测试。 （C）2002 Elsevier Science B.V.和国际化学疗法学会。版权所有。 [参考：27]

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