...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>International journal of antimicrobial agents >Prostatitis: what is the role of infection
【24h】

Prostatitis: what is the role of infection

机译：前列腺炎：感染有什么作用

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Although bacterial prostatitis is a common diagnosis, well documented infections of the prostate are uncommon. Culture studies of prostate tissue led our group to hypothesize that bacterial colonization/invasion of the prostate gland might occur more commonly than is appreciated by standard microbiological techniques. Specific polymerase chain reaction (PCR) assays were used for each of the pathogens previously implicated in chronic prostatitis as well as broad-spectrum PCR assays to identify tetracycline resistance genes and bacterial ribosomal-encoding genes (16S rDNAs), followed by cloning and sequencing of the PCR products. Only ten (8%) of the 135 patients with chronic prostatitis had positive specific PCR assays including: Mycoplasma genitalium in four men, Chlamydia trachomatis in three and Trichomonas vaginalis in two, as well as one man positive for both M. genitalium and C. trachomatis. In contrast to the specific probes, the broad-spectrum PCR assays had a substantial proportion of positives. We found evidence of tetracycline resistance in 25% of patients. 16S rDNA-encoding sequences in 77% of the subjects. The tetracycline resistance positives were a subset of the 16S rDNA positive patients. Patients with 16S rDNA-encoding sequences were significantly more likely to have expressed prostatic secretion leukocytes. Many patients with chronic prostatitis/chronic pelvic pain syndrome have a wide variety of bacterial DNA-encoding sequences despite extensive negative microbiological investigations. Understanding the precise role of infection in this syndrome may well lead to better methods to elucidate the microbiology of the prostate in health and disease. (C) 2002 Elsevier Science B.V. and International Society of Chemotherapy All rights reserved. [References: 35]
机译：尽管细菌性前列腺炎是一种常见的诊断方法，但有据可查的前列腺感染并不常见。前列腺组织的文化研究使我们的小组假设，细菌的定植/侵入前列腺可能比标准微生物学技术更普遍地发生。对于先前与慢性前列腺炎有关的每种病原体，均使用了特异性聚合酶链反应（PCR）分析法，以及广谱PCR分析法，以鉴定四环素抗性基因和细菌核糖体编码基因（16S rDNA），然后对其克隆和测序PCR产物。在135例慢性前列腺炎患者中，只有十（8％）的特异性PCR检测阳性，其中包括：四名男性生殖器支原体，三名沙眼衣原体和两名阴道毛滴虫，以及生殖器支原体和C阳性的一名人。沙眼与特定探针相反，广谱PCR分析具有很大比例的阳性。我们在25％的患者中发现了四环素耐药性的证据。 77％的受试者中的16S rDNA编码序列。四环素抗性阳性是16S rDNA阳性患者的一部分。具有16S rDNA编码序列的患者明显更有可能表达了前列腺分泌白细胞。尽管进行了广泛的阴性微生物研究，许多患有慢性前列腺炎/慢性盆腔疼痛综合征的患者仍具有多种细菌DNA编码序列。了解感染在该综合征中的确切作用很可能会导致更好的方法来阐明健康和疾病中前列腺的微生物学。 （C）2002 Elsevier Science B.V.和国际化学疗法学会保留所有权利。 [参考：35]

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号