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首页> 外文期刊>International Chickpea and Pigeonpea Newsletter >Induction of Morphological Mutants in Chickpea
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Induction of Morphological Mutants in Chickpea

机译：鹰嘴豆形态突变的诱导

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The study was aimed at enhancing the frequency and spectrum of mutations in chickpea (Cicer arietinum) through mutagenesis for achieving desired plant characteristics. Uniform and healthy seeds of two varieties of chickpea (Avrodhi and BG 256), presoaked in distilled water for 9 h, were treated with chemical mutagens, 0.1, 0.2, 0.3 and 0.4% of EMS (ethylmethane sulfonate) and 0.01, 0.02,0.03 and 0.04% of SA (sodium azide) and HZ (hydrazine hydrate) for 6 h. Solutions of all the three chemical mutagens were prepared in phosphate buffer of pH 7. For each treatment three hundred seeds were used. Treated seeds were sown in the field with three replications in a complete randomized block design, with each replication consisting of 100 seeds. Seeds soakedin distilled water were used as controls. Seeds of M_2 plants and control plants of both the varieties were harvested separately and sown in plant progeny rows to raise M_2. A wide range of morphological mutants were isolated in M_2 (Table 1). Mutationfrequency was estimated for each mutant in each variety and each treatment as percentage of the total M, plants. The frequency and spectrum of morphological mutants was relatively wide with EMS treatments followed by HZ and SA. The variety Avrodhi gave higher frequency of morphological mutants than BG 256 (Table 1). This reflects differences in their mutagenic sensitivity. The differential spectrum of morphological mutations has been reported in chickpea also by Kharkwal (1999). Most of the mutants, isolated in this study, exhibited negative selection value due to pleiotropic nature of the mutated genes. However, the compact growth mutant may be useful in chickpea breeding as experimental material for understanding the linkage relationships of genes.
机译：该研究旨在通过诱变提高鹰嘴豆（Cicer arietinum）突变的频率和频谱，以实现所需的植物特性。将两种鹰嘴豆（Avrodhi和BG 256）的均匀健康种子浸入蒸馏水中9 h，然后用化学诱变剂，0.1％，0.2％，0.3％和0.4％的EMS（乙基磺酸乙酯）和0.01、0.02、0.03进行处理。和0.04％的SA（叠氮化钠）和HZ（水合肼）反应6小时。在pH 7的磷酸盐缓冲液中制备所有三种化学诱变剂的溶液。每次处理均使用三百粒种子。在田间播种处理过的种子，以完全随机区组设计进行三次重复，每次重复由100粒种子组成。将种子苏克丁蒸馏水用作对照。分别收获两个品种的M_2植物和对照植物的种子，并将其播种在植物后代行中以培育M_2。在M_2中分离了多种形态突变体（表1）。估计每种变体和每种处理中每个突变体的突变频率占总植株的百分比。形态学突变体的频率和频谱相对较宽，其次是EMS处理，然后是HZ和SA。 Avrodhi品种比BG 256具有更高的形态突变率（表1）。这反映出其诱变敏感性的差异。 Kharkwal（1999）也曾在鹰嘴豆中报道过形态突变的差异谱。在本研究中分离出的大多数突变体由于突变基因的多效性而表现出负选择值。然而，紧凑型生长突变体可能在鹰嘴豆育种中用作理解基因之间的连锁关系的实验材料。

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