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首页> 外文期刊>International Journal of biological chemistry >Comparative Evaluation of Antimicrobial and Anti-Inflammatory Activities of Ocimum sanctum, Phyllanthus niruri and Cadaba fruticosa: An in vitro Approach with Emphasis on Detection of their Bioactive Compounds Using GC-MS
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Comparative Evaluation of Antimicrobial and Anti-Inflammatory Activities of Ocimum sanctum, Phyllanthus niruri and Cadaba fruticosa: An in vitro Approach with Emphasis on Detection of their Bioactive Compounds Using GC-MS

机译：比较评价乌头，余甘子和金叶果的抗菌和抗炎活性：重点是使用GC-MS检测其生物活性化合物的体外方法。

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摘要

The current investigation was performed with the objective of finding the scientific reason for health benefit of Occimum sanctum, Phyllanthus niruri and Cadaba fruticosa, simultaneously evaluating the antioxidant activity of ethanol, methanol and aqueous extracts. The potent antioxidant, antimicrobial and anti-inflammatory leaf extracts can be used for producing a novel ointment formulation, functional food or neutraceutical, in combination. The qualitative analysis of antioxidant activity was performed by reducing power assay and DPPH assay and in vitro anti-inflammatory test was performed by the HRBC membrane stabilization method. Further, the bioactive compounds present in the alcoholic leaf extracts were detected by GC-MS analysis and their applications were noted. Comparative studies on antimicrobial and anti-inflammatory activity of the 3 samples were determined using respective standards. Cadaba fruticosa is an under-emphasized herb, whose potential in the medical world is yet to be explored in details. Hence, Cadaba fruticosa has been chosen as one of the herbal plants for comparative study with Ocimum sp. and Phyllanthus sp. Ethanolic extract of showed significant antimicrobial activity against the tested bacterial strains. Methanolic extract of Ocimum sanctum at 200 mug mL~(-1) depicted highest scavenging activity, 81.1%. The results also indicate that Cadaba fruticosa possesses significant free radical scavenging and anti-inflammatory activities in vitro. GC-MS analysis of the herbs revealed the presence of several bioactive compounds.
机译：进行当前的研究的目的是找到科学的理由对圣殿，叶葵和金叶菊健康有益，同时评估乙醇，甲醇和水提取物的抗氧化活性。有效的抗氧化剂，抗微生物剂和抗炎叶提取物可用于组合生产新型软膏剂，功能性食品或营养保健品。通过降低功率测定法和DPPH测定法进行抗氧化活性的定性分析，并通过HRBC膜稳定化方法进行体外抗炎试验。此外，通过GC-MS分析检测了酒精性叶提取物中存在的生物活性化合物，并指出了它们的应用。使用各自的标准确定了3个样品的抗微生物和抗炎活性的比较研究。 Cadaba fruticosa是一种强调不足的草药，其在医学界的潜力尚待详细探讨。因此，Cadaba fruticosa已被选作与Ocimum sp。进行比较研究的草药植物之一。和Phyllanthus sp。乙醇提取物对测试的细菌菌株显示出显着的抗菌活性。罗汉果的甲醇提取物在200杯mL〜（-1）处表现出最高的清除活性，为81.1％。该结果还表明，金叶蝉在体外具有显着的自由基清除和抗炎活性。草药的GC-MS分析表明存在几种生物活性化合物。

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