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首页> 外文期刊>International Journal of Biological Macromolecules: Structure, Function and Interactions >Study on the purification and characterization of a polysaccharide conjugate from tea flowers
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Study on the purification and characterization of a polysaccharide conjugate from tea flowers

机译：茶花多糖共轭物的纯化与表征研究

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摘要

In this paper, the crude polysaccharides from the flowers of tea plant (Camellia sinensis) (TFPS) extracted with hot water were fractionated on a DEAE Sepharose FF chromatography to get TFPS1 with a yield of 18%. The properties and chemical compositions of TFPS1 were analyzed with GC, HPGPC, IC, IR methods and its morphology was observed with atomic force microscopy (AFM). The results showed that TFPS1 was a neutral glycoprotein conjugate with a molecular weight 500. kDa. The alanine, threonine, glycine, valine, serine, histidine, glutamic acid, histidine and tyrosine were found in TFPS1 and the total content was 2.03%. TFPS1 was consisted of rhamnose, arabinose, mannose, glucose and galactose, with a mole ratio of 1.0:2.9:0.5:1.3:3.3. Sugar backbone of TFPS1 may consist of glucose and galactose, but branched chain may consist of arabinose, galactose and rhamnose. The IR spectrum of TFPS1 revealed the typical characteristics of polysaccharides and protein. TFPS1 was spherical particle structure with a diameter of 50-70 nm.
机译：本文用热水提取的茶树（茶花）花（TFPS）中的粗多糖在DEAE Sepharose FF色谱上进行分馏，得到TFPS1，收率18％。用GC，HPGPC，IC，IR方法分析了TFPS1的性质和化学组成，并用原子力显微镜（AFM）观察了其形态。结果表明，TFPS1是分子量为500 kDa的中性糖蛋白结合物。在TFPS1中发现了丙氨酸，苏氨酸，甘氨酸，缬氨酸，丝氨酸，组氨酸，谷氨酸，组氨酸和酪氨酸，总含量为2.03％。 TFPS1由鼠李糖，阿拉伯糖，甘露糖，葡萄糖和半乳糖组成，摩尔比为1.0：2.9：0.5：1.3：3.3。 TFPS1的糖主链可能由葡萄糖和半乳糖组成，但支链可能由阿拉伯糖，半乳糖和鼠李糖组成。 TFPS1的红外光谱揭示了多糖和蛋白质的典型特征。 TFPS1为球形颗粒结构，直径为50-70 nm。

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