Evaluation of chitoligosaccharides effect upon probiotic bacteria

Fernandes J.C.; Eaton P.; Franco I.; Ramos ó.S.; Sousa S.; Nascimento H.; Gomes A.; Santos-Silva A.; Xavier Malcata F.; Pintado M.E.

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Evaluation of chitoligosaccharides effect upon probiotic bacteria

机译：壳寡糖对益生菌的作用评估

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The main objective of the present study was to evaluate the antibacterial effect - through the determination of minimum inhibitory (and lethal) concentrations, as well as the possible prebiotic potential of chitooligosaccharides (COS) - through the determination of growth curves, on Bifidobacterium animalis Bb12, Bifidobacterium animalis Bo and Lactobacillus acidophilus Ki. Atomic force microscopy was further used to obtain high resolution images of COS effects upon the cell morphology. Our results demonstrate that COS do not stimulate the growth of those strains, neither the strains are capable of using COS as a primary source of carbon. Analysis of morphology when exposed to inhibitory/bactericidal concentrations, suggested that COS do not exert any direct damage upon the bacteria structure, instead the bacteria are apparently covered by COS, which likely prevent nutrient uptake.

机译：本研究的主要目的是通过确定动物双歧杆菌Bb12的最小抑菌（和致死）浓度以及壳寡糖（COS）的可能益生元潜力来评估其抗菌作用。，动物双歧杆菌Bo和嗜酸乳杆菌Ki。原子力显微镜还用于获得COS效应对细胞形态的高分辨率图像。我们的结果表明，COS不会刺激这些菌株的生长，而且这些菌株都无法使用COS作为主要碳源。暴露于抑制/杀菌浓度下的形态分析表明，COS不会对细菌结构产生任何直接损害，相反，细菌显然被COS覆盖，这很可能会阻止营养物质的吸收。

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《International Journal of Biological Macromolecules: Structure, Function and Interactions》 |2012年第1期|共5页
作者
Fernandes J.C.; Eaton P.; Franco I.; Ramos ó.S.; Sousa S.; Nascimento H.; Gomes A.; Santos-Silva A.; Xavier Malcata F.; Pintado M.E.;
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正文语种 eng
中图分类生物大分子的结构和功能;
关键词
Atomic force microscopy; Chitooligosaccharides; Chitosan; Probiotic bacteria;

机译：原子力显微镜;壳寡糖;壳聚糖;益生菌;

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机译：壳寡糖对益生菌的作用评估
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Evaluation of chitoligosaccharides effect upon probiotic bacteria

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