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首页> 外文期刊>International Journal of Biological Macromolecules: Structure, Function and Interactions >Poly(hydroxyethyl methacrylate) based affinity membranes for in vitro removal of anti-dsDNA antibodies from SLE plasma
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Poly(hydroxyethyl methacrylate) based affinity membranes for in vitro removal of anti-dsDNA antibodies from SLE plasma

机译：基于聚甲基丙烯酸羟乙酯的亲和膜用于体外从SLE血浆中去除抗dsDNA抗体

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摘要

The preparation of polymeric membrane using affinity technology for application in blood filtration devices is described here. DNA attached poly(hydroxyethyl methacrylate) (PHEMA) based microporous affinity membrane was prepared for selective removal of anti-dsDNA antibodies from systemic lupus erythematosus (SLE) patient plasma in in vitro. In order to further increase blood-compatibility of affinity membrane, aminoacid based comonomer N-methacryloyl-l-alanine (MAAL) was included in the polymerization recipe. PHEMAAL membrane was produced by a photopolymerization technique and then characterized by swelling tests and scanning electron microscope (SEM) studies. Blood-compatibility tests were also performed. The water swelling ratio of PHEMAAL membrane increased significantly (133.2%) compared with PHEMA (58%). PHEMAAL membrane has large pores around in the range of 5-10μm. All the clotting times increased when compared with PHEMA membrane. Loss of platelets and leukocytes was very low. DNA loading was 7.8mg/g. There was a very low anti-dsDNA-antibody adsorption onto the plain PHEMAAL membrane, about 78 IU/g. The PHEMAAL-DNA membrane adsorbed anti-dsDNA-antibody in the range of 10-68×10~3IU/g from SLE plasma. Anti-dsDNA-antibody concentration decreased significantly from 875 to 144IU/ml with the time. Anti-dsDNA-antibodies could be repeatedly adsorbed and eluted without noticeable loss in the anti-dsDNA-antibody adsorption amount.
机译：本文描述了使用亲和技术制备用于血液过滤装置的聚合物膜。制备了基于DNA的聚甲基丙烯酸羟乙酯（PHEMA）基微孔亲和膜，用于在体外从系统性红斑狼疮（SLE）患者血浆中选择性去除抗dsDNA抗体。为了进一步提高亲和膜的血液相容性，在聚合配方中包括了基于氨基酸的共聚单体N-甲基丙烯酰基-1-丙氨酸（MAAL）。 PHEMAAL膜通过光聚合技术生产，然后通过溶胀测试和扫描电子显微镜（SEM）研究进行表征。还进行了血液相容性测试。与PHEMA（58％）相比，PHEMAAL膜的水溶胀率显着提高（133.2％）。 PHEMAAL膜在5-10μm范围内具有大孔。与PHEMA膜相比，所有凝血时间均增加。血小板和白细胞的损失非常低。 DNA负载为7.8mg / g。抗dsDNA抗体在PHEMAAL膜上的吸附非常低，约为78 IU / g。 SLE血浆中PHEMAAL-DNA膜吸附的抗dsDNA抗体为10-68×10〜3IU / g。随着时间的流逝，抗dsDNA抗体的浓度从875降至144IU / ml。可以反复吸附和洗脱抗-dsDNA-抗体，而抗-dsDNA-抗体的吸附量没有明显损失。

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