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首页> 外文期刊>International Journal of Biological Macromolecules: Structure, Function and Interactions >Sulfated modification, characterization and antitumor activities of Radix hedysari polysaccharide
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Sulfated modification, characterization and antitumor activities of Radix hedysari polysaccharide

机译：何首乌多糖的硫酸化修饰，表征及抗肿瘤活性

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摘要

Sulfated modification of a polysaccharide obtained from Radix hedysari (RHP) was studied. Four sulfated derivatives (RHPS) with variable degrees of substitution (DS) were obtained by the chlorosulfonic acid method with ionic liquids (ILs) as solvent and 4-dimethylaminopyridine (DMAP) as catalyst. The structures of RHPS were characterized by FT-IR spectra and ~(13)C NMR spectra, and the results indicated that the sulfated groups were modified mainly at the C-6 position and C-2 position. Four kinds of RHPS showed different DS ranging from 0.63 to 1.45, and different weight-average molecular mass (Mw) ranging from 60.8 to 71.1kDa with a little degradation. Compared with RHP, all of RHPS exhibited obvious antitumor activity on A549 cells and BGC-823 cells in vitro. However, they had no obvious influence on HEK293 cells, which indicated that they had low toxicity to normal cells. Flow cytometric studies indicated that the treatment of RHPS against A549 cells and BGC-823 cells could mediate the cell-cycle arrest in the G1 phase.
机译：研究了从七首乌（RHP）获得的多糖的硫酸化修饰。通过氯磺酸法，以离子液体（ILs）为溶剂，以4-二甲基氨基吡啶（DMAP）为催化剂，得到四种具有不同取代度（DS）的硫酸化衍生物（RHPS）。通过FT-IR光谱和〜（13）C NMR光谱对RHPS的结构进行了表征，结果表明硫酸化基团主要在C-6位和C-2位被修饰。四种RHPS的DS均在0.63至1.45之间，重均分子量（Mw）在60.8至71.1kDa之间，降解程度很小。与RHP相比，所有RHPS在体外对A549细胞和BGC-823细胞均表现出明显的抗肿瘤活性。但是，它们对HEK293细胞没有明显的影响，表明它们对正常细胞毒性低。流式细胞术研究表明，针对A549细胞和BGC-823细胞的RHPS处理可以介导G1期细胞周期停滞。

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