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首页> 外文期刊>International Journal of Biological Macromolecules: Structure, Function and Interactions >Characterization and optimization of schizophyllan production from date syrup
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Characterization and optimization of schizophyllan production from date syrup

机译：枣糖浆中裂枝多糖的表征与优化

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摘要

This study demonstrates the efficient utilization of low-cost agricultural substrates, particularly date syrup, by Schizophyllum commune ATCC 38548 for schizophyllan production. Initially, one factor-at a-time method was used to find the best carbon and nitrogen sources for schizophyllan production. Subsequently, response surface methodology was employed to optimize the level of culture medium components to maximize substrate conversion yield and schizophyllan production in submerged culture. Maximum product yield (0.12 g schizophyllan/g date syrup) and schizophyllan production (8.5 g/l) were obtained at concentrations of date syrup and corn steep liquor, inoculum size and agitation rate at 7.02 %w/v, 0.10 %w/v, 7.68 %v/v and 181 rpm, respectively. Sugar composition analysis, FTIR, NMR and molar mass determination revealed the purity and molecular properties of recovered schizophyllan produced from date syrup as glycosidic linkage analysis showed three main schizophyllan characteristic peaks arising from the 3-linked, 3,6-linked and terminal glucose residues. Finally, process economic analysis suggested that use of date syrup and corn steep liquor as nutrients would result in approximately 6-fold reduction in cost of raw materials for schizophyllan production as compared to conventional carbon and nitrogen sources such as sucrose and malt extract. (C) 2016 Elsevier B.V. All rights reserved.
机译：这项研究表明，裂殖酵母公社ATCC 38548可有效利用低成本的农业底物，特别是大枣糖浆生产裂殖壶菌。最初，一次使用一种因子的方法来寻找用于生产裂褶多糖的最佳碳源和氮源。随后，采用响应面方法优化了培养基成分的水平，以最大程度地提高了沉浸培养中的底物转化率和裂殖壶菌的产量。在大枣糖浆和玉米浆的浓度，接种量和搅拌速率分别为7.02％w / v，0.10％w / v的情况下，获得了最大的产品产量（0.12 g裂褶多糖/ g枣糖浆）和schizophyllan产量（8.5 g / l）。 ，7.68％v / v和181 rpm。糖成分分析，FTIR，NMR和摩尔质量测定揭示了从日期糖浆中生产的回收裂殖体的纯度和分子特性，因为糖苷键连接分析显示了由3个连接的，3,6连接的和末端的葡萄糖残基引起的3个主要的裂殖体特征峰。最后，过程经济分析表明，与传统的碳源和氮源（如蔗糖和麦芽提取物）相比，使用大枣糖浆和玉米浆作为营养物质将使裂藻多糖的生产原料成本降低约6倍。 （C）2016 Elsevier B.V.保留所有权利。

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