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首页> 外文期刊>International Journal of Biological Macromolecules: Structure, Function and Interactions >On the molecular structure of the amylopectin fraction isolated from 'high-amylose' ae maize starches
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On the molecular structure of the amylopectin fraction isolated from 'high-amylose' ae maize starches

机译：从“高直链淀粉” ae玉米淀粉中分离支链淀粉部分的分子结构

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The amylopectin fractions from starch of a series of amylose-extender (ae) maize samples (HYLON (R) V, VII and VIII starches) were isolated and analysed for their molecular composition and structure. The fractions from all samples contained both a high and a low molecular weight fraction (HMF and LMF), of which LMF increased with the amylose content of the starch and appeared to have substantially more of long chains than HMF. A normal amylose-containing maize starch (NMS), which served as a reference sample, contained very little LMF, which suggested that LMF was the inherent result of the effect of the loss of starch branching enzyme IIb activity in the ae mutants. Clusters were isolated from the amylopectin fractions using Bacillus amyloliquefaciens alpha-amylase, which effectively hydrolyses long internal chain segments between clusters. During the hydrolysis process, clearly more of small dextrins were released from the ae starches in comparison to NMS. It appeared that some of these small dextrins did not precipitate in methanol together with the majority of the clusters. Nevertheless, isolated clusters from the HYLON starch samples were smaller than in NMS and the clusters possessed a lower density of branches with longer chains. The composition of small, branched building blocks was also clearly different; HYLON starch samples possessed much more of single-branched blocks and less multiple-branched blocks than NMS. (C) 2016 Elsevier B.V. All rights reserved.
机译：分离了一系列直链淀粉延长剂（ae）玉米样品（HYLON V，VII和VIII淀粉）的淀粉中的支链淀粉馏分，并对其分子组成和结构进行了分析。来自所有样品的级分均包含高分子量级分和低分子量级分（HMF和LMF），其中LMF随着淀粉中直链淀粉含量的增加而增加，并且长链比HMF明显多。用作参考样品的普通含直链淀粉的玉米淀粉（NMS）含有很少的LMF，这表明LMF是ae突变体中淀粉分支酶IIb活性丧失的内在结果。使用解淀粉芽孢杆菌α-淀粉酶从支链淀粉级分中分离出簇，其可有效水解簇之间的长内链节。在水解过程中，与NMS相比，显然从ae淀粉中释放出更多的小糊精。似乎其中一些小糊精并未与大多数簇一起在甲醇中沉淀。然而，从HYLON淀粉样品中分离出的簇比NMS中的簇小，并且簇具有较低的具有较长链的分支密度。分支的小构件的组成也明显不同。与NMS相比，HYLON淀粉样品具有更多的单分支嵌段和更少的多分支嵌段。 （C）2016 Elsevier B.V.保留所有权利。

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