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The investigation of novel glucose oxidase biosensor based on graphene-clay nanocomposite

机译:基于石墨烯-粘土纳米复合材料的新型葡萄糖氧化酶生物传感器的研究

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摘要

A feasible and fast method to fabricate glucose biosensor was developed by immobilisation of glucose oxidase (GOx) to nano-sepiolite-graphene nanocomposite/glassy carbon electrode. The direct electrochemistry of glucose oxidase not containing medioter and worked in air saturated condition for similarity of in vivo usage. The nano-sepiolite-graphene nanocomposite shows a high conductive and biocompatible template for enzyme immobilisation. The immobilised GOx on nanocomposite shows a couple of well defined reversible peak with the anodic and cathodic peak potentials of -430 and -460 mV (vs. Ag/AgCl), corresponding to the FAD/FADH_2 redox couple, and with a formal potential of -445 mV in 0.1 M phosphate buffer solution at pH = 7. The linear concentration rang of biosensor is 0.14-3.14 muM, and with detection limit of 0.04 uM. Also, the life time of biosensor is more than four months.
机译:通过将葡萄糖氧化酶(GOx)固定到纳米海泡石-石墨烯纳米复合材料/玻碳电极上,开发了一种可行的快速制备葡萄糖生物传感器的方法。葡萄糖氧化酶的直接电化学不包含中等水平,并且在空气饱和条件下工作,体内使用的相似性。纳米海泡石-石墨烯纳米复合材料显示了酶固定化的高导电性和生物相容性模板。固定在纳米复合材料上的GOx表现出一对定义明确的可逆峰,其阳极和阴极峰电位分别为-430和-460 mV(相对于Ag / AgCl),对应于FAD / FADH_2氧化还原对,其形式电位为在pH = 7的0.1 M磷酸盐缓冲溶液中为-445 mV。生物传感器的线性浓度范围为0.14-3.14μM,检测极限为0.04 uM。而且,生物传感器的寿命超过四个月。

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