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首页> 外文期刊>International journal of biomedical nanoscience and nanotechnology >Gene delivery to Jurkat T cells using non-viral vectors associated with magnetic nanoparticles
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Gene delivery to Jurkat T cells using non-viral vectors associated with magnetic nanoparticles

机译：使用与磁性纳米粒子相关的非病毒载体将基因传递至Jurkat T细胞

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摘要

This paper describes core-shell-type magnetic nanoparticles (MNPs) and magnetic lipoplexes, comprising these particles, formulated to efficiently transfect suspended human Jurkat leukaemia T cells upon application of a gradient magnetic field for magnetofection. Magnetofection of the Jurkat T cells using selected vector formulations resulted in a significant (up to 4.5-fold) enhancement in both luciferase reporter gene expression and the percentage of cells expressing eGFP, as compared to lipofection. Up to 27% of the Jurkat T cells were eGFP-positive as detected by fluorescence-activated cell sorting with correction for weak fluorescence of the lipid enhancer. The increased efficiency of magnetofection, as compared to lipofection, was shown to be at least partially attributable to increased cellular internalisation of the magnetic vectors upon magnetic field application, as compared to non-magnetic lipoplexes. The metabolic activity of the cells post-magnetofection was comparable to that of untreated cells, suggesting non-toxicity of the vector 48 h post-magnetofection.
机译：本文介绍了核壳型磁性纳米颗粒（MNP）和包含这些颗粒的磁性脂复合物，这些颗粒被配制为在应用梯度磁场进行磁转染时有效转染悬浮的Jurkat白血病T细胞。与脂转染相比，使用选定的载体制剂对Jurkat T细胞进行磁转染可导致萤光素酶报告基因表达和表达eGFP的细胞百分比显着提高（最多4.5倍）。如通过荧光激活细胞分选法检测到的那样，多达27％的Jurkat T细胞为eGFP阳性，并校正了脂质增强剂的弱荧光。与脂转染相比，磁转染效率的提高被证明至少部分归因于与非磁性脂复合物相比，磁场施加后磁性载体在细胞内的增加。磁转染后细胞的代谢活性与未处理的细胞相当，表明载体在磁转染后48小时无毒。

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