Quantum dot bioconjugates: emerging tools with great potential to study protein interactions and dynamics by FRET

Eva M. Galvez

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Quantum dot bioconjugates: emerging tools with great potential to study protein interactions and dynamics by FRET

机译：量子点生物共轭物：新兴的工具，具有通过FRET研究蛋白质相互作用和动力学的巨大潜力

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Methods based on fluorescence technology have been widely employed to analyse protein function, dynamics, interactions and folding in cell free systems as well as in intact cells. Fluorescence resonance energy transfer (FRET) is a unique technique to precisely calculate inter- and intra-molecular distances in proteins and thus to study protein-protein/peptide interaction, folding and conformational changes. However detailed studies of these processes at the single molecule level and/or in intact cells, requires fluorescence dyes with high stability and quantum yield. During the last six years fluorescent nanocrystals (quantum dots) have been developed as potent and promising fluorescence dyes that fulfil the prerequisites to study protein function in FRET based assays. It is the aim of this short review to summarise the benefits and drawbacks of QDs in the study of protein function and dynamics in biological systems.

机译：基于荧光技术的方法已广泛用于分析无细胞系统和完整细胞中的蛋白质功能，动力学，相互作用和折叠。荧光共振能量转移（FRET）是一种独特的技术，可以精确计算蛋白质中的分子间和分子内距离，从而研究蛋白质-蛋白质/肽相互作用，折叠和构象变化。然而，在单分子水平和/或完整细胞中对这些过程的详细研究需要具有高稳定性和量子产率的荧光染料。在过去的六年中，荧光纳米晶体（量子点）已被开发为有效而有前途的荧光染料，满足了在基于FRET的测定中研究蛋白质功能的先决条件。这篇简短综述的目的是总结QD在研究生物系统中蛋白质功能和动力学方面的优缺点。

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《International journal of biomedical nanoscience and nanotechnology》 |2011年第1期|共20页
作者
Eva M. Galvez;
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正文语种 eng
中图分类生物科学;
关键词
nanotechnology; quantum dots; proteins; fluorescence resonance energy transfer; FRET.;

机译：纳米技术;量子点;蛋白质;荧光共振能量转移;FRET;

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专利

1. Quantum dot bioconjugates: emerging tools with great potential to study protein interactions and dynamics by FRET [J] . Eva M. Galvez International journal of biomedical nanoscience and nanotechnology . 2011,第1期

机译：量子点生物共轭物：新兴的工具，具有通过FRET研究蛋白质相互作用和动力学的巨大潜力
2. Compact, Polyvalent Mannose Quantum Dots as Sensitive, Ratiometric FRET Probes for Multivalent Protein-Ligand Interactions [J] . Guo Yuan, Sakonsinsiri Chadamas, Nehlmeier Inga, Angewandte Chemie . 2016,第15期

机译：紧凑的多价甘露糖量子点，可作为灵敏的比例FRET探针，用于多价蛋白-配体相互作用
3. Quantum mechanics and molecular dynamics studies on the C center dot center dot center dot H interaction between small fullerenes (C-36 and C-24) and [M(H2O)(6)](2+) (M = Ca2+, Zn2+) cations [J] . Yaghoobi Fereshteh, Salehzadeh Sadegh, Maddah Mina Journal of Molecular Liquids . 2020,第1期

机译：小富勒烯（C-36和C-24）和[M（H2O）（6）]（2 +）（M = Ca2 +，Zn2 +）之间C中心点中心点中心点H相互作用的量子力学和分子动力学研究阳离子
4. Bioconjugates of luminescent CdSe-ZnS quantum dots with engineered recombinant proteins: Novel self-assembled tools for biosensing [C] . J. Matthew Mauro, George P. Anderson, Phan T. Tran, Symposium on Semiconductor Quantum Dots . 2001

机译：具有工程重组蛋白的发光Cdse-Zns量子点的生物缀合物：新型自组装工具用于生物传感
5. Study of protein-RNA interactions using fluorescence resonance energy transfer (FRET) and single-molecule FRET. [D] . Lamichhane, Rajan. 2011

机译：使用荧光共振能量转移（FRET）和单分子FRET研究蛋白质-RNA相互作用。
6. Compact Polyvalent Mannose Quantum Dots as Sensitive Ratiometric FRET Probes for Multivalent Protein–Ligand Interactions [O] . Dr. Yuan Guo, Chadamas Sakonsinsiri, Dr. Inga Nehlmeier, -1

机译：紧凑的多价甘露糖量子点作为灵敏的比例FRET探针用于多价蛋白-配体相互作用
7. Compact, Polyvalent Mannose Quantum Dots as Sensitive, Ratiometric FRET Probes for Multivalent Protein–Ligand Interactions [O] . Guy Yuan, Sakonsinsiri Chadmamas, Nehlmeier Inga, 2016

机译：紧凑的多价甘露糖量子点作为敏感的比率FRET探针，用于多价蛋白质 - 配体相互作用

Quantum dot bioconjugates: emerging tools with great potential to study protein interactions and dynamics by FRET

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