...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>International Journal of Cancer =: Journal International du Cancer >Human Merkel cell polyomavirus infection I. MCV T antigen expression in Merkel cell carcinoma, lymphoid tissues and lymphoid tumors.
【24h】

Human Merkel cell polyomavirus infection I. MCV T antigen expression in Merkel cell carcinoma, lymphoid tissues and lymphoid tumors.

机译：人类默克尔细胞多瘤病毒感染I. MCV T抗原在默克尔细胞癌，淋巴组织和淋巴瘤中的表达。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Merkel cell polyomavirus (MCV) is a recently discovered human virus closely related to African green monkey lymphotropic polyomavirus. MCV DNA is integrated in approximately 80% of Merkel cell carcinomas (MCC), a neuroendocrine skin cancer linked to lymphoid malignancies such as chronic lymphocytic leukemia (CLL). To assess MCV infection and its association with human diseases, we developed a monoclonal antibody that specifically recognizes endogenous and transfected MCV large T (LT) antigen. We show expression of MCV LT protein localized to nuclei of tumor cells from MCC having PCR quantified MCV genome at an average of 5.2 (range 0.8-14.3) T antigen DNA copies per cell. Expression of this putative viral oncoprotein in tumor cells provides the mechanistic underpinning supporting the notion that MCV causes a subset of MCC. In contrast, although 2.2% of 325 hematolymphoid malignancies surveyed also showed evidence for MCV infection by DNA PCR, none were positive at high viral copy numbers, and none of 173 lymphoid malignancies examined on tissue microarrays expressed MCV LT protein in tumor cells. As with some of the other human polyomaviruses, lymphocytes may serve as a tissue reservoir for MCV infection, but hematolymphoid malignancies associated with MCC are unlikely to be caused by MCV.
机译：默克尔细胞多瘤病毒（MCV）是最近发现的与非洲绿猴淋巴多瘤病毒密切相关的人类病毒。 MCV DNA整合在大约80％的默克尔细胞癌（MCC）中，默克尔细胞癌是与淋巴恶性肿瘤（例如慢性淋巴细胞性白血病（CLL））相关的神经内分泌皮肤癌。为了评估MCV感染及其与人类疾病的关系，我们开发了一种单克隆抗体，可特异性识别内源性和转染的MCV大T（LT）抗原。我们显示了MCV LT蛋白的表达，该蛋白定位于来自具有PCR定量MCV基因组的MCC的MCC肿瘤细胞核，平均每个细胞5.2（0.8-14.3）T抗原DNA拷贝。该推测的病毒癌蛋白在肿瘤细胞中的表达提供了机制基础，支持了MCV导致MCC子集的观点。相比之下，尽管在接受调查的325例血淋巴恶性肿瘤中有2.2％也显示了通过DNA PCR感染MCV的证据，但在高病毒拷贝数下均没有阳性，并且在组织微阵列上检查的173淋巴恶性肿瘤均未在肿瘤细胞中表达MCV LT蛋白。与其他一些人类多瘤病毒一样，淋巴细胞可能充当MCV感染的组织库，但是与MCC相关的血淋巴恶性肿瘤不太可能由MCV引起。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号