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首页> 外文期刊>International journal of clinical pharmacology and therapeutics >Methylation of quercetin and fisetin, flavonoids widely distributed in edible vegetables, fruits and wine, by human liver.
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Methylation of quercetin and fisetin, flavonoids widely distributed in edible vegetables, fruits and wine, by human liver.

机译：槲皮素和非瑟酮的甲基化，类黄酮被人体肝脏广泛分布在食用蔬菜，水果和葡萄酒中。

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The aim of this investigation was to study the methylation of quercetin and fisetin, 2 chemically related flavonoids, in human liver and to this purpose, an assay was set-up to measure the rates of quercetin and fisetin methylation in human liver. The methylation rates (pmol/min/mg) of quercetin and fisetin were measured in 10 liver samples and the mean +/- SD and the median were 170+/-30 and 177 (quercetin) and 183+/-15 and 178 (fisetin). The rates of quercetin and fisetin methylation were not different (p = 0.283). The fold of variation among samples was 2 (quercetin) and 1.3 (fisetin). Methyltransferase towards quercetin and fisetin followed Michaelis-Menten kinetics, and the Km values were 2.6+/-0.3 (quercetin) and 8.6+/-0.7 microM (fisetin, p = 0.009) and the Vmax values were 187+/-20 (quercetin) and 276+/-33 pmol/min/mg (fisetin, p = 0.009). Two, 4 and 8 microl of red Chianti wine added to the incubation mixture reduced the rate of quercetin methylation to 75+/-4%, 65+/-9% and 59+/-9%, respectively, and that of fisetin methylation to 62+/-3%, 51+/-3% and 44+/-4%, respectively. In conclusion, quercetin and fisetin are methylated in human liver and their rates of methylation have a limited variation among subjects.
机译：这项研究的目的是研究人肝中槲皮素和非瑟酮（两种化学相关的类黄酮）的甲基化，并为此目的建立了一种测定法，以测定人肝中槲皮素和非瑟汀的甲基化率。在10个肝样本中测定了槲皮素和非瑟汀的甲基化率（pmol / min / mg），平均值+/- SD和中位数分别为170 +/- 30和177（槲皮素）和183 +/- 15和178（非瑟汀）。槲皮素和非瑟汀甲基化率无差异（p = 0.283）。样品之间的变化倍数为2（槲皮素）和1.3（非瑟汀）。朝向槲皮素和菲塞汀的甲基转移酶遵循Michaelis-Menten动力学，Km值为2.6 +/- 0.3（槲皮素）和8.6 +/- 0.7 microM（菲塞汀，p = 0.009），Vmax值为187 +/- 20（槲皮素） ）和276 +/- 33 pmol / min / mg（非瑟汀，p = 0.009）。向培养混合物中加入2、4和8微升基安蒂红葡萄酒，槲皮素甲基化率分别降低到75 +/- 4％，65 +/- 9％和59 +/- 9％，而非瑟定甲基化率降低分别达到62 +/- 3％，51 +/- 3％和44 +/- 4％。总之，槲皮素和非瑟汀在人肝中被甲基化，并且它们的甲基化速率在受试者之间变化有限。

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