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首页> 外文期刊>International journal of fertility and women's medicine >Comparison of three sperm preparation media.
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Comparison of three sperm preparation media.

机译：三种精子制备培养基的比较。

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OBJECTIVE: In assisted reproduction, spermatozoa must be effectively separated from seminal plasma to undergo capacitation, a prerequisite for fertilization. Percoll was recently withdrawn from the American market because of safety concerns. The purpose of this study was to evaluate the quality of sperm separated by two different sperm washing media, and compare these results to sperm quality after separation on a Percoll (Perwash) gradient. MATERIALS AND METHODS: Semen samples from 10 normozoospermic men were compared after separation on three media: Perwash, a Percoll-type medium (Conception Technologies, La Jolla, CA), ISolate (Irvine Scientific, Santa Ana, CA), and SpermFertil (Embryotech Laboratories, Wilmington, MA). Semen characteristics examined were: sperm count, percentage motility, curvilinear velocity, lateral head displacement, percentage recovery of motile sperm, viability, hypo-osmotic swelling, and penetration in bovine cervical mucus. Sperm morphology was scored using World Health Organization and Kruger's strict criteria. The motility of the sperm was examined at one-hour intervals for three hours to determine which method produced samples with the longest period of motility. RESULTS:Total motile sperm count, motility, curvilinear velocity, and percentage of normal morphological forms as determined by the WHO method were significantly lower in specimens prepared by SpermFertil than in ISolate or Perwash specimens (P < .05). Tail abnormalities were significantly more frequent in specimens prepared by SpermFertil than in ISolate and Perwash specimens (P < .001). Percentage recovery of motile sperm was significantly higher in ISolate and Perwash specimens than in SpermFertil specimens (P < .05). Semen characteristics were similar in specimens prepared with ISolate and with Perwash. At all time intervals, sperm motility was higher in ISolate and Perwash specimens than in SpermFertil specimens (P < .001). CONCLUSION: Sperm samples separated with a SpermFertil column have poorer sperm quality in several respects than samples prepared with ISolate or Perwash. This finding and the similarity between ISolate and Percoll procedures suggests that ISolate is a good alternative to Percoll-type media to prepare sperm for assisted reproduction.
机译：目的：在辅助生殖中，必须将精子与精浆有效分离以进行获能，这是受精的先决条件。由于安全方面的考虑，Percoll最近被撤出了美国市场。这项研究的目的是评估由两种不同的精子洗涤介质分离的精子的质量，并将这些结果与在Percoll（Perwash）梯度上分离后的精子质量进行比较。材料与方法：在10种正常精子症男性的精液样品中，分别在以下三种培养基上进行比较：Perwash，Percoll型培养基（Conception Technologies，La Jolla，CA），ISolate（Irvine Scientific，Santa Ana，CA）和SpermFertil（Embryotech）实验室，马萨诸塞州威尔明顿）。检查的精液特征为：精子数量，运动百分率，曲线速度，侧头移位，活动精子恢复百分率，生存力，低渗透性肿胀和在牛宫颈粘液中的渗透。使用世界卫生组织和克鲁格的严格标准对精子形态进行评分。每隔一小时检查一次精子的运动能力，持续三个小时，以确定哪种方法产生的运动时间最长。结果：用WHO方法测定的精子总运动精子数，运动性，曲线速度和正常形态的百分比显着低于ISolate或Perwash标本（P <.05）。用SpermFertil制备的标本中尾部异常的发生率显着高于使用ISolate和Perwash标本的组（P <.001）。 ISolate和Perwash标本中运动精子的回收率显着高于SpermFertil标本（P <.05）。用ISolate和Perwash制备的样品的精液特性相似。在所有时间间隔内，ISolate和Perwash标本中的精子活动性均高于SpermFertil标本中的精子运动性（P <.001）。结论：用SpermFertil色谱柱分离的精子样品在某些方面比用ISolate或Perwash制备的样品的精子质量差。这一发现以及ISolate和Percoll程序之间的相似性表明，ISolate是Percoll型培养基准备精子用于辅助生殖的良好替代方法。

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