• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>British journal of ophthalmology >VEGF localisation in diabetic retinopathy.
【24h】

VEGF localisation in diabetic retinopathy.

机译：糖尿病视网膜病变中的VEGF定位。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

AIM: To determine the staining pattern of vascular endothelial growth factor (VEGF) at different stages of diabetic retinopathy (including post-laser photocoagulation) and to compare staining in excised fibrovascular and fibrocellular (non-diabetic) preretinal membranes. METHODS: Immunohistochemical localisation of VEGF, using antibodies raised against VEGF165 and VEGF121,165,189, was carried out on specimens of normal human retina (n = 15), diabetic retinas ((a) with no overt retinopathy (n = 19), (b) with intraretinal vascular abnormalities but no proliferative retinopathy (n = 6), (c) with active proliferative retinopathy (n = 6), (d) with no residual proliferative retinopathy after photocoagulation therapy (n = 15)), excised diabetic fibrovascular membranes (n = 19), and non-diabetic fibrocellular membranes (n = 7). The degree and pattern of immunostaining was recorded. RESULTS: In general, VEGF was absent from the majority of normal retinas. VEGF staining was apparent in most diabetic tissues but the staining pattern was dependent on both the specificity of the antibody used and the category of tissue. Staining with the VEGF165 antibody was generally confined to endothelial cells adn perivascular regions while the VEGF121,165,189 antibody was also associated with extravascular components of the inner retina. Intensity of immunostaining of diabetic eyes was dependent on the severity of retinopathy being least in diabetics with no overt retinopathy and greatest in retinas with proliferative retinopathy. Interestingly, the intensity of immunostaining in diabetic retinas which had undergone laser surgery for proliferative retinopathy was reduced to basal levels. Moderate to intense immunostaining was observed in all fibrovascular and fibrocellular membranes examined. CONCLUSIONS: This study supports a circumstantial role for VEGF in the pathogenesis of both the preclinical and proliferative stages of diabetic retinopathy.
机译：目的：确定糖尿病性视网膜病变（包括激光后光凝）不同阶段的血管内皮生长因子（VEGF）的染色模式，并比较切除的血管和纤维细胞（非糖尿病）视网膜前膜的染色。方法：使用针对VEGF165和VEGF121,165,189的抗体对正常人视网膜（n = 15），糖尿病视网膜（（a）没有明显的视网膜病变（n = 19），（b）标本进行了VEGF的免疫组织化学定位。 ）视网膜内血管异常但无增生性视网膜病变（n = 6），（c）活跃性增生性视网膜病变（n = 6），（d）光凝治疗后无残留增生性视网膜病变（n = 15）），切除的糖尿病性纤维膜（n = 19）和非糖尿病性纤维细胞膜（n = 7）。记录免疫染色的程度和模式。结果：一般而言，大多数正常视网膜不存在VEGF。在大多数糖尿病组织中，VEGF染色很明显，但是染色模式取决于所用抗体的特异性和组织类别。用VEGF165抗体染色通常局限于内皮细胞和血管周围区域，而VEGF121,165,189抗体也与内视网膜的血管外成分有关。糖尿病眼的免疫染色强度取决于视网膜病变的严重程度，在没有明显视网膜病变的糖尿病患者中最小，而在具有增殖性视网膜病变的视网膜中最大。有趣的是，已经对接受了增生性视网膜病变的激光手术的糖尿病视网膜的免疫染色强度降低到了基础水平。在检查的所有纤维血管和纤维细胞膜中均观察到中度至强烈的免疫染色。结论：本研究支持在糖尿病性视网膜病的临床前和增殖期的发病机理中VEGF的间接作用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号