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首页> 外文期刊>International Journal of Food Microbiology >Combined effects of benomyl and environmental factors on growth and expression of the fumonisin biosynthetic genes FUM1 and FUM19 by Fusarium verticillioides
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Combined effects of benomyl and environmental factors on growth and expression of the fumonisin biosynthetic genes FUM1 and FUM19 by Fusarium verticillioides

机译：苯菌灵和环境因子对褐藻镰刀菌生长伏马菌素生物合成基因FUM1和FUM19的联合影响

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摘要

Fusarium verticillioides is predominantly responsible of fumonisin contamination of maize and other cereals in Mediterranean climatic regions. This study examined the interaction of the fungicide benomyl, at ED50 and ED90 concentrations (effective doses of benomyl to reduce growth by 50% and 90%, respectively), with a range of temperatures (20-35 degrees C) and water potentials (-0.7, -2.8 and -7.0 MPa) compatible with current and foreseen climate change scenarios for these regions on growth and fumonisin biosynthesis in in vitro assays. The expression of fumonisin biosynthetic genes (FUM1 and FUM19) was quantified by real time RT-PCR. FUM1 encodes a polyketide synthase and FUM19 an ABC-type transporter, located both in the fumonisin biosynthetic cluster. The ED50 and ED90 concentrations obtained at 25 degrees C were 0.93 mg/L and 3.30 mg/L, respectively. Benomyl affected growth and fumonisin gene expression differently but it generally reduced fungal growth and fumonisin biosynthesis and both were significantly affected by temperature and water potential, This indicated that efficacy of benomyl might be compromised at certain conditions, although at similar or lower levels than other fungicides tested. Both fumonisin biosynthetic genes had similar expression patterns in all treatments and their correlation was positive and significant The results suggested that Mediterranean climatic scenarios might suffer an additional negative impact of climate change by reducing the efficacy of antifungals used to control pathogens and toxigenic fungi. (C) 2014 Elsevier B.V. All rights reserved.
机译：verticillioides镰刀菌是造成地中海气候地区玉米和其他谷物伏马菌素污染的主要原因。这项研究研究了在一定温度范围（20-35摄氏度）和水势（-）下，在ED50和ED90浓度（有效剂量的苯菌灵分别使生长减少50％和90％）下杀菌剂苯菌灵的相互作用。 0.7，-2.8和-7.0 MPa）与这些区域在体外测定中生长和伏马菌素生物合成的当前和可预见的气候变化情景兼容。伏马菌素生物合成基因（FUM1和FUM19）的表达通过实时RT-PCR定量。 FUM1编码一个聚酮化合物合酶，而FUM19一个ABC型转运蛋白，都位于伏马毒素生物合成簇中。在25摄氏度下获得的ED50和ED90浓度分别为0.93 mg / L和3.30 mg / L。苯菌灵对生长和伏马菌素基因表达的影响不同，但通常会降低真菌的生长和伏马菌素的生物合成，并且都受到温度和水势的显着影响。这表明苯菌灵的功效在某些条件下可能会受到影响，尽管其水平与其他杀菌剂相似或更低。经过测试。伏马菌素的两个生物合成基因在所有处理中均具有相似的表达模式，且它们的相关性呈正相关且显着性。结果表明，通过降低用于控制病原体和产毒真菌的抗真菌药的功效，地中海气候情景可能会遭受气候变化的其他负面影响。 （C）2014 Elsevier B.V.保留所有权利。

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