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首页> 外文期刊>International journal of gynecological cancer: official journal of the International Gynecological Cancer Society >Expression of matrix metalloproteinase-2 and mutant p53 is increased in hydatidiform mole as compared with normal placenta.
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Expression of matrix metalloproteinase-2 and mutant p53 is increased in hydatidiform mole as compared with normal placenta.

机译：与正常胎盘相比，葡萄胎中葡萄胎基质金属蛋白酶2和突变体p53的表达增加。

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摘要

Matrix metalloproteinases (MMPs) are group of enzymes thought to play an important role in trophoblastic and tumor invasion. The aim of our study was to investigate the trophoblastic expression of MMPs and p53 in normal trophoblast and hydatidiform moles (HM). Paraffin sections of 45 specimens, including 14 complete hydatidiform moles (CM), 15 partial hydatidiform moles (PM), 8 atypical partial hydatidiform moles (aPM), and 8 controls were selected. Classification of HM was established on histologic criteria and supported by the DNA ploidy results. Tissue sections from each case were immunostained with monoclonal antibodies, cytokeratin-7, MMP-2, MMP-9, tissue inhibitors of metalloproteinases (TIMP)-1, and p53 wild type (p53wt) and mutant types (mutp53). Staining for cytokeratin-7 revealed a positive reaction in 93% of the samples. MMP-2 was mainly expressed in the syncytiotrophoblast of HM and found in 62% of aPM, 60% PM, and 93% CM. The mutp53 was mainly and focally expressed in syncytiotrophoblasticcells and was found in 63% of aPM, 80% PM, and 93% CM. Expression of MMP-2 and mutp53 was both significantly greater in HM vs control group (P < 0.05) and greater in CM vs PM and aPM (P < 0.05). No significant difference was observed for cytokeratin-7, MMP-9, TIMP-1, and p53wt between the HM subgroups and between HM and control group. MMP-2 and mutp53 are overexpressed in HM as compared with normal trophoblast and might participate in the invasive behavior of the HM.
机译：基质金属蛋白酶（MMP）是一类被认为在滋养细胞和肿瘤侵袭中起重要作用的酶。我们研究的目的是研究正常滋养细胞和葡萄胎（HM）中MMP和p53的滋养细胞表达。选择了45个标本的石蜡切片，包括14个完整的葡萄胎（CM），15个葡萄胎（PM），8个非典型葡萄胎（aPM）和8个对照。 HM的分类是根据组织学标准建立的，并得到DNA倍性结果的支持。用单克隆抗体，细胞角蛋白7，MMP-2，MMP-9，金属蛋白酶组织抑制剂（TIMP）-1和p53野生型（p53wt）和突变型（mutp53）对每种病例的组织切片进行免疫染色。细胞角蛋白7染色显示93％的样品呈阳性反应。 MMP-2主要在HM的合体滋养层中表达，在62％的aPM，60％的PM和93％的CM中发现。 mutp53主要并集中在合体滋养层细胞中，在63％的aPM，80％的PM和93％的CM中发现。 HM组与对照组相比，MMP-2和mutp53的表达均显着较高（P <0.05），而CM与PM和aPM的表达均较高（P <0.05）。在HM亚组之间以及HM与对照组之间，未观察到细胞角蛋白7，MMP-9，TIMP-1和p53wt的显着差异。与正常滋养细胞相比，MMP-2和mutp53在HM中过表达，可能参与HM的侵袭行为。

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