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首页> 外文期刊>Biochemical Genetics >Microsatellite markers to determine population genetic structure in the golden anchovy, Coilia dussumieri
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Microsatellite markers to determine population genetic structure in the golden anchovy, Coilia dussumieri

机译：微卫星标记确定金an鱼（Coilia dussumieri）中的种群遗传结构

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摘要

Coilia dussumieri (Valenciennes, 1848) commonly called as golden anchovy, constitutes a considerable fishery in the northern part of both the west and east coasts of India. Despite its clear-cut geographic isolation, the species is treated as a unit stock for fishery management purposes. We evaluated 32 microsatellite primer pairs from three closely related species (resource species) belonging to the family Engraulidae through cross-species amplification in C. dussumieri. Successful cross-priming was obtained with 10 loci, which were sequenced for confirmation of repeats. Loci were tested for delineating the genetic stock structure of four populations of C. dussumieri from both the coasts of India. The number of alleles per locus ranged from 8 to 18, with a mean of 12.3. Results of pairwise FST indicated genetic stock structuring between the east and west coast populations of India and also validated the utilization of identified microsatellite markers in population genetic structure analysis.
机译：Coilia dussumieri（Valenciennes，1848）通常被称为黄金凤尾鱼，在印度西海岸和东海岸的北部构成大量的渔业。尽管地理上明显地孤立，但出于渔业管理目的，该物种被视为单位种群。我们通过C. dussumieri中的跨物种扩增，评估了来自属于Engraulidae科的三个密切相关物种（资源物种）的32个微卫星引物对。用10个基因座获得了成功的交叉引物，将其测序以确认重复。测试了基因座，以描绘来自印度两个海岸的四个杜氏梭菌种群的遗传种群结构。每个基因座的等位基因数量为8至18，平均为12.3。成对FST的结果表明了印度东西海岸人口之间的遗传种群结构，也验证了已鉴定的微卫星标记在人口遗传结构分析中的利用。

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