Construction of Full-Length cDNA Library and Development of EST-Derived Simple Sequence Repeat (EST-SSR) Markers in Senecio scandens

Qian Gang; Ping Junjiao; Lu Jian; Zhang Zhen; Wang Lei; Xu Delin

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Construction of Full-Length cDNA Library and Development of EST-Derived Simple Sequence Repeat (EST-SSR) Markers in Senecio scandens

机译：千里光树中全长cDNA文库的构建和EST衍生的简单序列重复（EST-SSR）标记的开发。

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Senecio scandens Buch.-Ham. ex D. Don (Compositae) is a crucial source of Chinese traditional medicine with antibacterial properties. We constructed a cDNA library and obtained expressed sequence tags (ESTs) to show the distribution of gene ontology annotations for mRNAs, using an individual plant with superior antibacterial characteristics. Analysis of comparative genomics indicates that the putative uncharacterized proteins (21.07%) might be derived from "molecular function unknown" clones or rare transcripts. Furthermore, the Compositae had high cross-species transferability of EST-derived simple sequence repeats (EST-SSR), based on valid amplifications of 206 primer pairs developed from the newly assembled expressed sequence tag sequences in Artemisia annua L. Among those EST-SSR markers, 52 primers showed polymorphic amplifications between individuals with contrasting diverse antibacterial traits. Our sequence data and molecular markers will be cost-effective tools for further studies such as genome annotation, molecular breeding, and novel transcript profiles within Compositae species.

机译：塞内西奥（Senecio）scandens Buch.-Ham。前D.唐（菊科）是具有抗菌特性的中药的重要来源。我们构建了一个cDNA文库，并使用具有优异抗菌特性的单株植物获得了表达的序列标签（EST），以显示mRNA的基因本体注释的分布。对比较基因组学的分析表明，推定的未表征蛋白质（21.07％）可能来自“分子功能未知”克隆或罕见转录本。此外，基于从青蒿中新组装的表达序列标签序列开发的206个引物对的有效扩增，菊科植物具有很高的跨物种转移性，可从EST衍生简单序列重复序列（EST-SSR）。在这些EST-SSR中标记，52个引物显示了个体之间具有不同抗菌特性的多态性扩增。我们的序列数据和分子标记将是用于进一步研究的经济有效的工具，例如基因组注释，分子育种以及菊科物种内的新转录本概况。

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《Biochemical Genetics》 |2014年第12期|共15页
作者
Qian Gang; Ping Junjiao; Lu Jian; Zhang Zhen; Wang Lei; Xu Delin;
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正文语种 eng
中图分类遗传学;
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Senecio scandens Buch.-Ham. ex D. Don; Expressed sequence tag (EST); Full-length cDNA library; Simple sequence repeat (SSR);

机译：塞内西奥（Senecio）scandens Buch.-Ham。 ex D. Don;表达序列标签（EST）;全长cDNA文库;简单序列重复（SSR）;

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1. 橡胶树 EST-SSR标记开发及特性研究 [J] . 李德军, 邓治, 郭会娜, 农业科学与技术（英文版） . 2014,第005期
2. Construction of Full-Length cDNA Library and Development of EST-Derived Simple Sequence Repeat (EST-SSR) Markers in Senecio scandens [J] . Qian Gang, Ping Junjiao, Lu Jian, Biochemical Genetics . 2014,第11a12期

机译：千里光树中全长cDNA文库的构建和EST衍生的简单序列重复（EST-SSR）标记的开发。
3. Development and characterization of simple sequence repeat (SSR) markers based on a full-length cDNA library of Napier Grass (Pennisetum purpureum Schum) [J] . Wang Jia, Chen Zhitong, Jin Surong, Genes and genomics . 2017,第12期

机译：基于Napier Grass全长cDNA文库的简单序列重复（SSR）标记的开发与表征（Pennisetum purpureum schum）
4. Development and characterization of simple sequence repeat (SSR) markers based on a full-length cDNA library of Scutellaria baicalensis [J] . Yuan Yuan, Long Ping, Jiang Chao, Genomics . 2015,第1期

机译：基于黄S全长cDNA文库的简单序列重复（SSR）标记的开发和表征
5. Development of Polymorphic Simple Sequence Repeat Markers in Lilium regale by Magnesphere Method [C] . Y.P.Zhao, W.H.Wang, G.S.Liu, International conference on germplasm of ornamentals . 2013

机译：Magnesphere方法在椴树升温中进行多态性简单序列重复标记的发展
6. Development of simple sequence repeat markers for bermudagrass from bermudagrass EST sequences and preexisting sorghum SSR markers. [D] . Tan, Chengcheng. 2009

机译：从百慕大草EST序列和已有的高粱SSR标记开发了百慕大的简单序列重复标记。
7. Expressed Sequence Tags Analysis and Design of Simple Sequence Repeats Markers from a Full-Length cDNA Library in Perilla frutescens (L.) [O] . Eun Soo Seong, Ji Hye Yoo, Jae Hoo Choi, 2015

机译：紫苏（L.）中全长cDNA文库的表达序列标签分析和简单序列重复标记的设计
8. Development, Characterization and Cross-species Transferability of Expressed Sequence Tag-simple Sequence Repeat (EST-SSR) Markers Derived from Kelampayan Tree Transcriptome [O] . P.S. Lai, W.S. Ho, S.L. Pang 2013

机译：表达序列标签 - 简单序列重复（EST-SSR）标记的显影，表征和交叉物种可转移性来自Kelampayan树转录组

Construction of Full-Length cDNA Library and Development of EST-Derived Simple Sequence Repeat (EST-SSR) Markers in Senecio scandens

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