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首页> 外文期刊>British journal of ophthalmology >The effect of postmortem time, donor age and sex on the generation of neurospheres from adult human retina.
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The effect of postmortem time, donor age and sex on the generation of neurospheres from adult human retina.

机译：验尸时间，供体年龄和性别对成年人类视网膜神经球生成的影响。

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BACKGROUND/AIM: Postmortem adult human retina contains pluripotent progenitor cells capable of forming neurospheres with different retinal cell types. The authors examine whether this is the case at all ages and at different postmortem times. METHODS: Adult human postmortem retina-derived cell suspensions generated neurospheres in fibroblast growth factor 2 and N2 supplement. The yield of neurospheres from limited dilution or single cell cultures is very low so the authors studied cells generated per 10(5) viable cells from a cell suspension derived from whole retina. Retinal tissue from donors aged 18-91 at various postmortem times (between 23-44 h) was studied in the context of generation rate and time for neurospheres. RESULTS: The potential to generate neurospheres from adult human retina remains throughout life. Neurosphere cellular components were not affected by donor age or postmortem time (they contained nestin(+), glial fibrillary acidic protein(+) and neurofilament(+) cells). An average of 34.36 neurospheres were generated per 10(5) viable cells. After a few days in culture neurospheres begin to form. The time for this to occur was independent of donor age but prolonged at longer postmortem times. No significant effect of donor sex was found. CONCLUSION: Neurosphere-forming retinal progenitor cells are found in adult human retina throughout life. This cell population is a potential target for therapeutic intervention to influence repair and regeneration of the retina.
机译：背景/目的：死后成年人类视网膜含有能够形成具有不同视网膜细胞类型的神经球的多能祖细胞。作者检查了是否在所有年龄段和不同的死后时间都是如此。方法：成年人类视网膜后细胞悬浮液在成纤维细胞生长因子2和N2补充物中产生了神经球。有限稀释或单细胞培养物中神经球的产量非常低，因此作者研究了每10（5）个活细胞从整个视网膜衍生的细胞悬液中产生的细胞。在神经球的产生速率和时间的背景下，研究了在不同的死后时间（23-44小时之间）年龄在18-91岁的供体的视网膜组织。结果：从成年人类视网膜产生神经球的潜力终生存在。神经球细胞成分不受供体年龄或死后时间的影响（它们含有巢蛋白（+），神经胶质纤维酸性蛋白（+）和神经丝（+）细胞）。每10（5）个活细胞平均产生34.36个神经球。培养几天后，神经球开始形成。发生时间与献血者的年龄无关，但在更长的死后时间会延长。未发现供体性别的显着影响。结论：成年人的视网膜终生发现形成神经球的视网膜祖细胞。该细胞群是治疗干预以影响视网膜修复和再生的潜在靶标。

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