...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>International journal of immunogenetics >The Pekin duck IL-10R2 common chain: CDNA cloning, genomic structure, molecular characterization and mRNA expression analysis
【24h】

The Pekin duck IL-10R2 common chain: CDNA cloning, genomic structure, molecular characterization and mRNA expression analysis

机译：北京鸭IL-10R2共有链：CDNA克隆，基因组结构，分子表征和mRNA表达分析

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Summary: The interleukin-10 receptor 2 (IL-10R2, IL-10Rβ) is required for the signalling of the class 2 cytokines IL-10, IL-22, IL-26 and IFN-λ1-3. Here, we describe the identification of the Pekin duck IL-10R2 (duIL-10R2) common chain and its gene structure. The duIL-10R2 cDNA encodes a 343 amino acid protein that has an amino acid identity of 76% and 42% with chicken and human IL-10R2, respectively. Binding residues of human IL-10R2 for IL-10 and IL-22 were mostly conserved in the avian IL-10R2 proteins within loops L3 and L5, but not within loops L2 and L6. Homology modelling of the duIL-10R2 extracellular domain structure using soluble human IL-10R2 (shIL-10R2, PDB ID: 3LQM) as a template revealed a protruding loop L5 and two distinct clefts between loops L2/L3 and L3/L5, similar to shIL-10R2. However, in contrast to the three amino acid β-hairpin loop L2 of shIL-10R2, loop L2 of duIL-10R2 is five residues longer. Residues within a putative Tyk2 binding site were highly conserved across all vertebrate IL-10R2 proteins examined. The duIL-10R2 gene shares a seven exon-six intron structure with chicken and human IL-10R2 genes, but avian genes are more compact. DuIL-10R2 mRNA was constitutively expressed in all tissues. Mitogen stimulation of duck peripheral blood mononuclear cells (PBMC) did not alter transcript levels. Our observations suggest that genomic organization and structural features implicated in multiple cytokine-binding properties of human IL-10R2 are conserved in duck IL-10R2, but the evolutionary changes that appear to have lead to low-affinity cytokine interaction within loop L2 are distinct to mammalian species.
机译：摘要：白介素10受体2（IL-10R2，IL-10Rβ）是2类细胞因子IL-10，IL-22，IL-26和IFN-λ1-3信号转导所必需的。在这里，我们描述了北京鸭IL-10R2（duIL-10R2）共同链的鉴定及其基因结构。 duIL-10R2 cDNA编码一个343个氨基酸的蛋白质，与鸡和人的IL-10R2的氨基酸同一性分别为76％和42％。人IL-10R2与IL-10和IL-22的结合残基在环L3和L5内的禽类IL-10R2蛋白中最保守，但在环L2和L6内不是。使用可溶性人IL-10R2（shIL-10R2，PDB ID：3LQM）作为模板对duIL-10R2细胞外域结构进行同源性建模，显示了一个突出的环L5和环L2 / L3和L3 / L5之间的两个明显裂口，类似于shIL-10R2。然而，与shIL-10R2的三个氨基酸β-发夹环L2相反，duIL-10R2的环L2长五个残基。在所有检测到的脊椎动物IL-10R2蛋白中，推定的Tyk2结合位点内的残基高度保守。 duIL-10R2基因与鸡和人IL-10R2基因共有7个外显子6内含子结构，但禽类基因更为紧凑。 DuIL-10R2 mRNA在所有组织中组成性表达。鸭外周血单核细胞（PBMC）的丝裂原刺激不会改变转录水平。我们的观察结果表明，与人IL-10R2的多种细胞因子结合特性有关的基因组组织和结构特征在鸭IL-10R2中是保守的，但似乎导致L2环内低亲和力细胞因子相互作用的进化变化与哺乳动物。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号