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首页> 外文期刊>International journal of immunogenetics >Isolation, polymorphism and expression study of two distinct major histocompatibility complex class II B genes from half-smooth tongue sole (Cynoglossus semilaevis).
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Isolation, polymorphism and expression study of two distinct major histocompatibility complex class II B genes from half-smooth tongue sole (Cynoglossus semilaevis).

机译：半光滑舌（Cynoglossus semilaevis）的两个不同的主要组织相容性复杂IIB类基因的分离，多态性和表达研究。

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Major histocompatibility complex (MHC) class II antigens are important in vertebrate immune system, which present peptides to CD4(+) T cells. In the present study, cDNAs encoding MHC class II B gene were isolated from the cDNA library of half-smooth tongue sole (Cynoglossus semilaevis), and the full length cDNA sequences were got by rapid amplification of cDNA ends polymerase chain reaction. The polymorphism of its open reading frame, 3' untranslated region and intron 1 was studied. Nineteen class II B alleles were identified from nine individuals and clustered into two groups, designated as Cyse-DAB and Cyse-DBB. The deduced amino acid sequences among Cyse-DAB and Cyse-DBB alleles shared identities from 94.0% to 99.6% and 92.4% to 99.6%, respectively, while the identities between Cyse-DAB and Cyse-DBB genes varied from 85.1% to 92.0%. Three Cyse-DAB alleles and one Cyse-DBB allele were observed in each of two individuals, and three Cyse-DBB alleles and one Cyse-DAB allele in another individual, which indicated at least two loci existed in each gene. Two different 3' UTR sequences were identified and one belonged to Cyse-DAB, the other belonged to Cyse-DBB. Both five Cyse-DAB and five Cyse-DBB intron 1 sequences were identified from genomic sequences, among which two sequences of each gene were identified in a single individual, which suggested the existence of at least two Cyse-DAB and two Cyse-DBB loci. Both the two genes'-specific tissue and developmental stage expression were studied by reverse-transcription polymerase chain reaction, which showed that the two genes had similar expression patterns in tissue study with high expression in spleen and kidney, low expression in liver, gill and ovary, moderate expression in brain, heart, intestine and testis. While in developmental analysis, Cyse-DBB had higher expression than Cyse-DAB in early developmental stages, which indicated that the two genes might have different functions in those stages. Therefore, in half-smooth tongue sole, two different MHC class II B genes exist and could differentiate from each other whether by sequence analysis or by developmental stage expression study.
机译：主要的组织相容性复合体（MHC）II类抗原在脊椎动物免疫系统中很重要，该系统可将肽呈递给CD4（+）T细胞。本研究从半光滑舌M（Cynoglossus semilaevis）的cDNA文库中分离出编码MHC II B类基因的cDNA，并通过快速扩增cDNA末端聚合酶链反应获得了全长cDNA序列。研究了其开放阅读框，3'非翻译区和内含子1的多态性。从9个个体中鉴定出19个II B类等位基因，并分为两组，分别称为Cyse-DAB和Cyse-DBB。 Cyse-DAB和Cyse-DBB等位基因推导的氨基酸序列的同一性分别为94.0％至99.6％和92.4％至99.6％，而Cyse-DAB和Cyse-DBB等位基因之间的同一性从85.1％至92.0％ 。在两个个体中分别观察到三个Cyse-DAB等位基因和一个Cyse-DBB等位基因，在另一个个体中观察到三个Cyse-DBB等位基因和一个Cyse-DAB等位基因，这表明每个基因中至少存在两个基因座。鉴定出两个不同的3'UTR序列，一个属于Cyse-DAB，另一个属于Cyse-DBB。从基因组序列中鉴定出五个Cyse-DAB和五个Cyse-DBB内含子1序列，其中在单个个体中鉴定了每个基因的两个序列，这表明存在至少两个Cyse-DAB和两个Cyse-DBB基因座。通过逆转录聚合酶链反应研究了这两个基因的特异性组织和发育阶段的表达，结果表明这两个基因在组织研究中具有相似的表达模式，在脾肾中高表达，在肝，g和肝中低表达。卵巢，在脑，心脏，肠和睾丸中有中等表达。在发育分析中，Cyse-DBB在早期发育阶段的表达高于Cyse-DAB，这表明这两个基因在这些阶段可能具有不同的功能。因此，在半光滑的舌底中，存在两个不同的MHC IIB类基因，并且可以通过序列分析或发育阶段表达研究彼此区分。

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