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首页> 外文期刊>Biochemical Genetics >Identification, inheritance, and variation of microsatellite markers in the black scallop Mimachlamys varia.
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Identification, inheritance, and variation of microsatellite markers in the black scallop Mimachlamys varia.

机译：黑色扇贝Mimachlamys varia中微卫星标记的鉴定，遗传和变异。

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摘要

Five polymorphic microsatellite loci were identified in the black scallop Mimachlamys varia after construction of a genomic library enriched for (GT)n. To examine the transmission pattern of microsatellite alleles, several families were created and genotypes scored for three loci. The expected Mendelian ratios were found in 12 of 14 segregations examined. Unexpected segregations may be explained by a genotyping error (allelic dropout), given that when a specific allele was treated as dominant, the phenotypic ratios conformed to Mendelian expectations. The five loci were also examined in two samples from the Spanish coast. The two localities displayed similar mean values for the number of alleles per locus (7.2-8.4), allelic richness (7.2-7.9), and observed (0.389-0.484) and expected heterozygosity (0.545-0.618). Significant Hardy-Weinberg deviations were observed at three loci, with heterozygote deficiency occurring in all cases. Global multilocus theta value and allele frequencies at one locus revealed significant differentiation between the two localities.
机译：构建富集（GT）n的基因组文库后，在黑色扇贝Mimachlamys varia中鉴定出五个多态微卫星基因座。为了检查微卫星等位基因的传播模式，创建了几个家族，并对三个位点的基因型进行了评分。在检查的14个隔离区中的12个隔离区中找到了预期的孟德尔比率。意外的隔离可能是由于基因分型错误（等位基因缺失）所致，因为当将特定等位基因视为优势基因时，表型比例符合孟德尔的预期。还从西班牙海岸的两个样本中检查了五个基因座。这两个位置显示每个位点的等位基因数量（7.2-8.4），等位基因丰富度（7.2-7.9）和观察到的（0.389-0.484）和预期的杂合度（0.545-0.618）的平均值均相近。在三个位点均观察到明显的Hardy-Weinberg偏差，在所有情况下均发生杂合子缺陷。一个位点的全局多位点theta值和等位基因频率揭示了两个位置之间的显着差异。

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