...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>International journal of immunopharmacology >Proteosome delivery of a protective 9B-antigen against Schistosoma mansoni.
【24h】

Proteosome delivery of a protective 9B-antigen against Schistosoma mansoni.

机译：抵抗曼氏血吸虫的保护性9B抗原的蛋白酶体递送。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

We have previously characterized a stage specific, partially protective protein denoted 9B-antigen. This antigen is of 450 kDa in its native form but upon SDS-PAGE in reducing conditions it exhibits two subunits of 30 kDa and 45 kDa. The 9B-antigen is localized at the surface of schistosomula and persists at the surface of lung schistosomula. The 9B-antigen is also localized in internal organs of a vital function in the parasite such as flame cells and cytoplasmic tubes. Infected individuals or mice vaccinated with irradiated cercariae recognize the 9B-antigen. We have previously shown that when injected with complete Freunds adjuvant, the 9B-antigen can induce 40% protection against challenge infection. In this study we have used a more effective delivery system for this antigen. The 9B-antigen was coupled to proteosomes derived from meningoccocal outer membrane proteins. Vaccination of mice with this complex increased the protection level to 60%. Sera from these vaccinated mice induced high levels of complement mediated cytotoxicity of the parasite. Since the proteosomes are approved for human use, these results are promising towards the development of a vaccine against schistosomiasis.
机译：我们先前已表征了阶段特异性的部分保护性蛋白质，称为9B抗原。该抗原的天然形式为450 kDa，但在还原条件下进行SDS-PAGE时，它表现出30 kDa和45 kDa的两个亚基。 9B抗原位于血吸虫的表面，并持续存在于肺血吸虫的表面。 9B抗原也位于该寄生虫的重要功能内部器官中，例如火焰细胞和细胞质管。接种了辐射尾尾的感染个体或小鼠识别9B抗原。先前我们已经表明，当注射完整的弗氏佐剂时，9B抗原可诱导40％的抗感染感染保护。在这项研究中，我们为这种抗原使用了更有效的递送系统。 9B-抗原与源自脑膜炎球菌外膜蛋白的蛋白体偶联。用这种复合物对小鼠进行疫苗接种可将保护水平提高到60％。这些接种疫苗的小鼠的血清诱导了高水平的补体介导的寄生虫的细胞毒性。由于蛋白体被批准用于人类，因此这些结果对于开发抗血吸虫病疫苗很有希望。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号