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首页> 外文期刊>International journal of immunogenetics >Molecular characterization of classical and nonclassical MHC class I genes from the golden pheasant (Chrysolophus pictus)
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Molecular characterization of classical and nonclassical MHC class I genes from the golden pheasant (Chrysolophus pictus)

机译：黄金phe（Chrysolophus pictus）的经典和非经典MHC I类基因的分子表征

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Classical major histocompatibility complex (MHC) class I allelic polymorphism is essential for competent antigen presentation. To improve the genotyping efforts in the golden pheasant, it is necessary to differentiate more accurately between classical and nonclassical class I molecules. In our study, all MHC class I genes were isolated from one golden pheasant based on two overlapping PCR amplifications. In total, six full-length class I nucleotide sequences (A-F) were identified, and four were novel. Two (A and C) belonged to the IA1 gene, two (B and D) were alleles derived from the IA2 gene through transgene amplification, and two (E and F) comprised a third novel locus, IA3 that was excluded from the core region of the golden pheasant MHC-B. IA1 and IA2 exhibited the broad expression profiles characteristic of classical loci, while IA3 showed no expression in multiple tissues and was therefore defined as a nonclassical gene. Phylogenetic analysis indicated that the three IA genes in the golden pheasant share a much closer evolutionary relationship than the corresponding sequences in other galliform species. This observation was consistent with high sequence similarity among them, which likely arises from the homogenizing effect of recombination. Our careful distinction between the classical and nonclassical MHC class I genes in the golden pheasant lays the foundation for developing locus-specific genotyping and establishing a good molecular marker system of classical MHC I loci.
机译：经典的主要组织相容性复合体（MHC）I类等位基因多态性对于有效的抗原呈递至关重要。为了改善golden鸡的基因分型工作，有必要更准确地区分经典I类分子和非经典I类分子。在我们的研究中，所有MHC I类基因都是基于两次重叠PCR扩增从一个锦鸡身上分离得到的。总共鉴定出六个全长的I类核苷酸序列（A-F），其中四个是新颖的。两个（A和C）属于IA1基因，两个（B和D）是通过转基因扩增从IA2基因衍生的等位基因，两个（E和F）包含第三个新基因座IA3，被排除在核心区域之外锦鸡MHC-B。 IA1和IA2展示了经典基因座的广泛表达谱特征，而IA3在多个组织中没有表达，因此被定义为非经典基因。系统发育分析表明，与其他鸡形目物种的相应序列相比，金鸡的三个IA基因具有更紧密的进化关系。该观察结果与它们之间的高序列相似性是一致的，这可能是由于重组的均质化作用引起的。我们对金鸡的经典MHC I类基因和非经典MHC I类基因的仔细区分，为开发基因座特异性基因分型和建立经典MHC I基因座的良好分子标记体系奠定了基础。

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