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首页> 外文期刊>Biochemical Genetics >Vicilin genes of Vigna luteola: structure, organization, expression, and variation.
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Vicilin genes of Vigna luteola: structure, organization, expression, and variation.

机译：Vigna luteola的Vicilin基因：结构，组织，表达和变异。

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摘要

Two different but related sequences that encode Vigna luteola 7S vicilins were isolated and characterized. The sequences differ by two nucleotide substitutions, each of which results in an amino acid replacement. This low level of divergence suggests that a recent gene duplication has occurred. Both variants are expressed in cDNA populations; therefore, neither gene is a pseudogene. Both copies were present in all individuals (72) analyzed using real-time PCR and TaqMan probes. Segregation was not observed. The two sequences are not independent alleles. Vicilin genomic sequences of 11 specimens from six geographic locations were determined. No polymorphic sites were identified in either of the two gene copies. This lack of polymorphism suggests that either a population bottleneck or selection has occurred. The genetic structure, expression patterns, and protein composition of the V. luteola vicilins were compared to those of other legume vicilins.
机译：分离并鉴定了编码Vigna luteola 7S vicilins的两个不同但相关的序列。序列的区别在于两个核苷酸取代，每个取代都会导致氨基酸取代。这种低水平的差异表明最近发生了基因重复。两种变体都在cDNA群体中表达。因此，两个基因都不是假基因。使用实时PCR和TaqMan探针分析的所有个体（72）中均存在两个拷贝。没有观察到偏析。这两个序列不是独立的等位基因。确定了来自六个地理位置的11个标本的Vicilin基因组序列。在两个基因拷贝的任何一个中均未鉴定出多态性位点。这种缺乏多态性的现象表明，种群瓶颈或选择已经发生。比较了黄豆堇菜丝球菌毒素的遗传结构，表达模式和蛋白质组成，并与其他豆类豌豆丝球菌毒素进行了比较。

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