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首页> 外文期刊>British Journal of Radiology >Dose estimation by chromosome aberration analysis and micronucleus assays in victims accidentally exposed to (60)Co radiation.
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Dose estimation by chromosome aberration analysis and micronucleus assays in victims accidentally exposed to (60)Co radiation.

机译：通过意外暴露于（60）Co辐射的受害者的染色体像差分析和微核分析进行剂量估算。

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The objective of this study was to assess the radiation exposure levels in victims of a (60)Co radiation accident using chromosome aberration analysis and the micronucleus assay. Peripheral blood samples were collected from three victims exposed to (60)Co 10 days after the accident and were used for the chromosome aberration and micronucleus assays. After in vitro culture of the lymphocytes, the frequencies of dicentric chromosomes and rings (dic+r) and the numbers of cytokinesis blocking micronuclei (CBMN) in the first mitotic division were determined and used to estimate radiation dosimetry. The Poisson distribution of the frequency of dic+r in lymphocytes was used to assess the uniformity of the exposure to (60)Co radiation. Based on the frequency of dic+r in lymphocytes, estimates of radiation exposure of the three victims were 5.61 Gy (A), 2.48 Gy (B) and 2.68 Gy (C). The values were estimated based on the frequencies of CBMN, which were 5.45 Gy (A), 2.78 Gy (B) and 2.84 Gy (C). The estimated radiation dosimetry demonstrated a critical role in estimating the radiation dose and facilitating an accurate clinical diagnosis. Furthermore, the frequencies of dir+r in victims A and B deviated significantly from a normal Poisson distribution. Chromosome aberration analysis offers a reliable means for estimating biological exposure to radiation. In the present study, the micronucleus assay demonstrated a high correlation with the chromosome aberration analysis in determining the radiation dosimetry 10 days after radiation exposure.
机译：这项研究的目的是使用染色体像差分析和微核分析法评估（60）Co辐射事故受害者的辐射暴露水平。事故发生十天后，从三名暴露于（60）Co的受害者中收集了外周血样本，并将其用于染色体畸变和微核分析。淋巴细胞体外培养后，确定有丝分裂第一个分裂中双着丝粒染色体和环的频率（dic + r）以及胞质分裂阻滞微核（CBMN）的数量，并用于估算辐射剂量。淋巴细胞中dic + r频率的泊松分布用于评估暴露于（60）Co辐射的均匀性。根据淋巴细胞中dic + r的频率，三个受害者的放射线暴露估计分别为5.61 Gy（A），2.48 Gy（B）和2.68 Gy（C）。该值是基于CBMN的频率估算的，分别为5.45 Gy（A），2.78 Gy（B）和2.84 Gy（C）。估计的放射剂量证明了在估计放射剂量和促进准确的临床诊断中的关键作用。此外，受害人A和B中dir + r的频率与正态Poisson分布显着不同。染色体畸变分析提供了一种可靠的方法来估计生物对辐射的暴露。在本研究中，微核试验证明在辐射暴露后10天确定辐射剂量时与染色体畸变分析高度相关。

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