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Prevalence of Anaplasma and Ehrlichia species in Ixodes persulcatus ticks and small mammals from different regions of the Asian part of Russia

机译:来自俄罗斯亚洲部分不同地区的Persulcatus壁虱tick虫和小型哺乳动物的无浆虫和埃希氏菌属流行

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A total 834 Ixodes persulcatus ticks and 641 small mammals from the areas of northern and southern Ural, Southeastern and Southwestern Siberia, and the Far East was examined on the presence of Anaplasma spp. and Ehrlichia spp. by nested PCR based on 16S rRNA gene with subsequent sequencing. A. phagocytophilum and E. muris DNAs were found in samples from ticks and small mammals in all studied areas. A. phagocytophilum DNA was revealed in 0.3-5.1% of ticks and 3.2-16.6% of small mammals from different regions, whereas E. muris DNA was found in 4.9-15.3% of ticks and 4.1-12.6% of small mammals. One L persulcatus tick and one east-European field vole from the Siberian region were shown to contain 'Candidatus Neoehrlichia mikurensis' DNA. A novel Ehrlichia species, closely related to Ehrlichia sp. EHf669. Ehrlichia sp. EH727, Ehrlichia sp. Tibet and 'Candidatus Ehrlichia shimanensis', was found using molecular techniques in tissue samples of 14.4% of small rodents from the Far East. (C) 2008 Elsevier GmbH. All rights reserved.
机译:检验了来自乌拉尔北部和南部,西伯利亚东南部和西南部以及远东地区的总共834个Persulcatus s和641个小型哺乳动物,并检查了无形体物种。和埃希氏菌属。通过基于16S rRNA基因的巢式PCR进行测序。在所有研究区域的壁虱和小型哺乳动物的样品中均发现了噬菌嗜酸杆菌和鼠埃里希氏菌DNA。来自不同地区的0.3-5.1%的s和3.2-16.6%的小型哺乳动物发现了嗜高曲霉DNA,而tick的4.9-15.3%和4.1-12.6%的mu鼠大肠杆菌DNA被发现。来自西伯利亚地区的一只L persulcatus壁虱和一只东欧野田鼠含有'Candidatus Neoehrlichia mikurensis'DNA。一种新的埃希氏菌属物种,与埃希氏菌属密切相关。 EHf669。埃里希氏菌EH727,埃里希氏菌属。使用分子技术在远东地区14.4%的小型啮齿动物的组织样本中发现了西藏和'Candidatus Ehrlichia shimanensis'。 (C)2008 Elsevier GmbH。版权所有。

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