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首页> 外文期刊>International Journal of Mycobacteriology >The draft genome of Mycobacterium aurum, a potential model organism for investigating drugs against Mycobacterium tuberculosis and Mycobacterium leprae
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The draft genome of Mycobacterium aurum, a potential model organism for investigating drugs against Mycobacterium tuberculosis and Mycobacterium leprae

机译：金黄色分枝杆菌的基因组草案，一种潜在的模型生物，可用于研究抗结核分枝杆菌和麻风分枝杆菌的药物

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摘要

Mycobacterium aurum (M. aurum) is an environmental mycobacteria that has previously been used in studies of anti-mycobacterial drugs due to its fast growth rate and low pathogenicity. The M. aurum genome has been sequenced and assembled into 46 contigs, with a total length of 6.02 Mb containing 5684 annotated protein-coding genes. A phyloge-netic analysis using whole genome alignments positioned M. aurum close to Mycobacterium uaccae and Mycobacterium vanbaalemi, within a clade related to fast-growing mycobacteria. Large-scale genomic rearrangements were identified by comparing the M. aurum genome to those of Mycobacterium tuberculosis and Mycobacterium leprae. M. aurum orthologous genes implicated in resistance to anti-tuberculosis drugs in M. tuberculosis were observed. The sequence identity at the DNA level varied from 68.6% for pncA (pyraz-inamide drug-related) to 96.2% for rrs (streptomycin, capreomycin). We observed two homologous genes encoding the catalase-peroxidase enzyme (featG) that isassociated with resistance to isoniazid. Similarly, two embB homologues were identified in the M. aurum genome. In addition to describing for the first time the genome of M. aurum, this work provides a resource to aid the use of M. aurum in studies to develop improved drugs for the pathogenic mycobacteria M. tuberculosis and M. leprae.
机译：金色分枝杆菌（M. aurum）是一种环境分枝杆菌，由于其快速生长和低致病性，以前已被用于抗分枝杆菌药物的研究。金黄色葡萄球菌的基因组已测序并组装成46个重叠群，全长6.02 Mb，其中包含5684个带注释的蛋白质编码基因。使用全基因组比对的系统遗传学分析将金黄色葡萄球菌放置在与快速增长的分枝杆菌有关的进化枝中靠近分枝杆菌和范分枝杆菌的地方。通过比较金黄色葡萄球菌基因组与结核分枝杆菌和麻风分枝杆菌的基因组，可以确定大规模的基因组重排。在结核分枝杆菌中观察到与抗结核药耐药有关的金黄色葡萄球菌直系同源基因。 DNA水平上的序列同一性从pncA（与吡嗪酰胺有关的药物）的68.6％到rrs（链霉素，卡普霉素）的96.2％不等。我们观察到两个编码过氧化氢酶-过氧化物酶（featG）的同源基因，它们与对异烟肼的抗性相关。类似地，在金黄色葡萄球菌基因组中鉴定了两个embB同源物。除了首次描述金黄色葡萄球菌的基因组外，这项工作还提供了资源，以协助金黄色葡萄球菌在研究中用于开发用于致病性分枝杆菌结核分枝杆菌和麻风分支杆菌的药物。

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