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首页> 外文期刊>International Journal of Radiation Biology: Covering the Physical, Chemical, Biological, and Medical Effects of Ionizing and Non-ionizing Radiations >Increased γ-H2AX by exposure to a 60-Hz magnetic fields combined with ionizing radiation, but not hydrogen peroxide, in non-tumorigenic human cell lines
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Increased γ-H2AX by exposure to a 60-Hz magnetic fields combined with ionizing radiation, but not hydrogen peroxide, in non-tumorigenic human cell lines

机译：在非致瘤性人类细胞系中，通过暴露于60 Hz磁场并结合电离辐射而不是过氧化氢，可增加γ-H2AX

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Purpose: Genotoxic effects have been considered the gold standard to determine if an environmental factor is a carcinogen, but the currently available data for extremely low frequency time-varying magnetic fields (ELF-MF) remain controversial. As an environmental stimulus, the effect of ELF-MF on cellular DNA may be subtle. Therefore, a more sensitive method and systematic research strategy are warranted to evaluate genotoxicity. Materials and methods: We investigated the effect of ELF-MF in combination with ionizing radiation (IR) or H2O2 on the DNA damage response of expression of phosphorylated H2AX (γ-H2AX) and production of γ-H2AX foci in non-tumorigenic human cell systems consisting of human lung fibroblast WI-38 cells and human lung epithelial L132 cells. Results: Exposure to a 60-Hz, 2 mT ELF-MF for 6 h produced increased γ-H2AX expression, as well as γ-H2AX foci production, a common DNA double-strand break (DSB) marker. However, exposure to a 1 mT ELF-MF did not have the same effect. Moreover, 2 mT ELF-MF exposure potentiated the expression of γ-H2AX and γ-H2AX foci production when combined with IR, but not when combined with H 2O2. Conclusions: ELF-MF could affect the DNA damage response and, in combination with different stimuli, provide different effects on γ-H2AX.
机译：目的：遗传毒性作用已被认为是确定环境因素是否为致癌物的金标准，但有关极低频时变磁场（ELF-MF）的当前可用数据仍存在争议。作为环境刺激，ELF-MF对细胞DNA的作用可能微妙。因此，有必要采用更灵敏的方法和系统的研究策略来评估遗传毒性。材料和方法：我们研究了ELF-MF与电离辐射（IR）或H2O2结合对非致瘤性人类细胞中磷酸化H2AX（γ-H2AX）表达和γ-H2AX灶产生的DNA损伤反应的影响系统由人肺成纤维细胞WI-38细胞和人肺上皮L132细胞组成。结果：暴露于60 Hz，2 mT ELF-MF中6 h会增加γ-H2AX表达，并增加γ-H2AX灶的产生，这是一种常见的DNA双链断裂（DSB）标记。但是，暴露于1 mT ELF-MF效果不一样。此外，2 mT ELF-MF暴露在与IR结合时增强了γ-H2AX和γ-H2AX病灶产生的表达，而与H 2O2结合则没有。结论：ELF-MF可以影响DNA损伤反应，并结合不同的刺激对γ-H2AX具有不同的作用。

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