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首页> 外文期刊>International Journal of Radiation Biology: Covering the Physical, Chemical, Biological, and Medical Effects of Ionizing and Non-ionizing Radiations >Effects of the exposure to intermittent 1.8 GHz radio frequency electromagnetic fields on HSP70 expression and MAPK signaling pathways in PC12 cells
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Effects of the exposure to intermittent 1.8 GHz radio frequency electromagnetic fields on HSP70 expression and MAPK signaling pathways in PC12 cells

机译：暴露于间歇性1.8 GHz射频电磁场对PC12细胞中HSP70表达和MAPK信号通路的影响

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Purpose: We previously reported effects on heat shock protein 70 (HSP70) mRNA expression, a cytoprotective protein induced under stressful condition, in human trophoblast cells exposed to amplitude-modulated Global System for Mobile Communication (GSM) signals. In the present work the same experimental conditions were applied to the rat PC12 cells, in order to assess the stress responses mediated by HSP70 and by the Mitogen Activated Protein Kinases (MAPK) in neuronal-like cells, an interesting model to study possible effects of mobile phone frequencies exposure. Materials and methods: HSP70 gene expression level was evaluated by reverse transcriptase polymerase chain reaction, HSP70 protein expression and MAPK phosphorylation were assessed by Western blotting. PC12 cells were exposed for 4, 16 or 24 h to 1.8 GHz continuous wave signal (CW, carrier frequency without modulation) or to two different GSM modulation schemes, GSM-217Hz and GSM-Talk (which generates temporal changes between two different GSM signals, active during talking or listening phases, respectively, thus simulating a typical conversation). Specific adsorption rate (SAR) was 2 W/kg. Results: After PC12 cells exposure to the GSM-217Hz signal for 16 or 24 h, HSP70 transcription significantly increased, whereas no effect was observed in cells exposed to the CW or GSM-Talk signals. HSP70 protein expression and three different MAPK signaling pathways were not affected by the exposure to any of the three different 1.8 GHz signals. Conclusion: The positive effect on HSP70 mRNA expression, observed only in cells exposed to the GSM-217Hz signal, is a repeatable response previously reported in human trophoblast cells and now confirmed in PC12 cells. Further investigations towards a possible role of 1.8 GHz signal modulation are therefore advisable.
机译：目的：我们先前报道了在暴露于振幅调制的全球移动通信系统（GSM）信号的人类滋养细胞中，热应激蛋白70（HSP70）mRNA表达的作用，该蛋白是在应激条件下诱导的细胞保护蛋白。在当前的工作中，将相同的实验条件应用于大鼠PC12细胞，以评估神经元样细胞中HSP70和丝裂原活化蛋白激酶（MAPK）介导的应激反应，这是一个有趣的模型，用于研究神经胶质细胞的可能作用。手机频率曝光。材料与方法：通过逆转录聚合酶链反应评估HSP70基因的表达水平，通过Western印迹法评估HSP70蛋白的表达和MAPK磷酸化。 PC12电池暴露4、16或24小时，暴露于1.8 GHz连续波信号（CW，无调制的载波频率）或暴露于两种不同的GSM调制方案GSM-217Hz和GSM-Talk（它们在两种不同的GSM信号之间产生时间变化） （分别在通话或收听阶段处于活动状态，从而模拟典型的对话）。比吸附率（SAR）为2W / kg。结果：PC12细胞暴露于GSM-217Hz信号16或24 h后，HSP70转录显着增加，而暴露于CW或GSM-Talk信号的细胞中未观察到影响。 HSP70蛋白表达和三个不同的MAPK信号通路不受暴露于三个不同的1.8 GHz信号中的任何一个的影响。结论：仅在暴露于GSM-217Hz信号的细胞中观察到对HSP70 mRNA表达的积极作用，这是以前在人类滋养细胞中报道的可重复反应，现在在PC12细胞中得到证实。因此，建议进一步研究1.8 GHz信号调制的可能作用。

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