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首页> 外文期刊>International Journal of Radiation Biology: Covering the Physical, Chemical, Biological, and Medical Effects of Ionizing and Non-ionizing Radiations >Radioprotective effect of amifostine on cells from cancer prone patients and healthy individuals studied by the G2 and PCC assays.
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Radioprotective effect of amifostine on cells from cancer prone patients and healthy individuals studied by the G2 and PCC assays.

机译：通过G2和PCC分析研究了氨磷汀对易癌患者和健康个体细胞的辐射防护作用。

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PURPOSE: To investigate whether amifostine is effective at reducing the yield of chromatid breaks when present during G(2)-phase irradiation of human normal cells and cells from cancer prone patients, as well as to study the mechanisms underlying the radioprotective effect of amifostine. MATERIALS AND METHODS: G(2) chromosomal radiosensitivity in the presence or absence of amifostine was studied in healthy donors, cancer patients, ataxia-telangietasia (A-T) patients and five human lymphoblastoid cell lines with genes predisposing to cancer. The yield of chromatid breaks following gamma-irradiation in G(2) phase was obtained at the subsequent metaphase using the G(2) assay. For scoring chromatid damage directly in G(2) or G(0) phase, premature chromosome condensation was used. RESULTS: When amifostine was present during irradiation, the mean yield of radiation-induced chromatid breaks as visualized by the G(2) assay was significantly reduced in healthy donors (t-test, p=0.001), in cells from cancer patients (p=0.001) and in cell lines from patients with genes predisposing to cancer (p=0.01) except ATM(-/-) (0.1<0.2). However, when chromatid breaks were scored directly in G(2) or G(0) phase using premature chromosome condensation, the presence of amifostine did not affect the yields obtained. CONCLUSION: Amifostine reduces the mean yield of chromatid breaks in normal cells and in cells from cancer prone patients when present during G(2) irradiation. Although the precise mechanisms of radioprotection caused by amifostine remain unclear, the results obtained using premature chromosome condensation reveal that amifostine does not act on cells only as a free radical scavenger and as a repair enhancer of DNA damage.
机译：目的：调查在人类正常细胞和易癌患者细胞的G（2）期照射过程中存在时，氨磷汀是否能有效降低染色单体断裂的产生，并研究氨磷汀的辐射防护作用机理。材料与方法：在健康捐献者，癌症患者，共济失调毛细血管扩张症（A-T）患者和五种具有易患癌基因的人类成淋巴细胞样细胞系中研究了存在或不存在氨磷汀的G（2）染色体放射敏感性。在G（2）相中进行伽马射线辐照后，染色质断裂的产量在随后的中期使用G（2）分析获得。为了直接在G（2）或G（0）相中评估染色单体损伤，使用了过早的染色体凝结。结果：当在放射期间存在氨磷汀时，健康供体的t（t检验，p = 0.001）和癌症患者的细胞中（p-0.001），通过G（2）分析可见，辐射诱导的染色单体断裂的平均产率显着降低。 = 0.001）和ATM（-/-）（0.1 <0.2）以外的具有易患癌症基因的患者的细胞系中（p = 0.01）。但是，当使用过早的染色体浓缩直接在G（2）或G（0）相中对染色单体断裂进行评分时，氨磷汀的存在不会影响所获得的产量。结论：氨磷汀在G（2）照射期间存在时，会降低正常细胞和易癌患者细胞中染色单体断裂的平均产量。尽管尚不清楚由氨磷汀引起的放射防护的确切机制，但使用过早的染色体凝结所获得的结果表明，氨磷汀不仅仅作为自由基清除剂和DNA损伤的修复促进剂而作用于细胞。

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