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首页> 外文期刊>British Poultry Science >Proton-coupled folate transporter (PCFT): molecular cloning, tissue expression patterns and the effects of dietary folate supplementation on mRNA expression in laying hens
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Proton-coupled folate transporter (PCFT): molecular cloning, tissue expression patterns and the effects of dietary folate supplementation on mRNA expression in laying hens

机译：质子偶联叶酸转运蛋白（PCFT）：分子克隆，组织表达模式和日粮叶酸补充对蛋鸡mRNA表达的影响

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摘要

1. The aim was to investigate the molecular characterisation and effects of dietary folate supplementation on mRNA concentrations of the proton-coupled folate transporter (PCFT) in chicken. 2. Twenty-four (n = 8/treatment) laying hens were allocated at random to one of three dietary treatments: a basal diet without supplemental folate, or the same diet with either 10 center dot 00 mg/kg folic acid or 11 center dot 30 (equimolar) mg/kg 5-methyltetrahydrofolate (5-MTHF) for 21 d. The PCFT cDNA was cloned using the TA cloning system, and mRNA concentrations were determined by real-time polymerase chain reaction (PCR). 3. The PCFT cDNA (1643 bp) was cloned from the duodenum and its transcripts were widely distributed in chicken tissues. Duodenal and caecal PCFT mRNA was not affected by dietary treatments; however, jejunal PCFT mRNA was significantly reduced in hens fed the 5-MTHF diet versus the basal diet. 4. A broad tissue distribution of PCFT transcripts is indicative of a vital role of PCFT in the folate transport in chicken. The expression of jejunal PCFT mRNA was down-regulated by dietary 5-MTHF supplementation. These findings contribute to an understanding of the development of folate transport and the PCFT system in avian systems involving laying hens.
机译：1.目的是研究日粮中叶酸补充剂对鸡质子偶联叶酸转运蛋白（PCFT）mRNA浓度的影响。 2. 24只（n = 8 /只）产蛋鸡随机分配给以下三种饮食疗法之一：基础饮食（不含补充叶酸）或相同的饮食（10中心点00 mg / kg叶酸或11中心）点30（等摩尔）mg / kg 5-甲基四氢叶酸（5-MTHF）进行21 d。使用TA克隆系统克隆PCFT cDNA，并通过实时聚合酶链反应（PCR）确定mRNA浓度。 3.从十二指肠克隆了PCFT cDNA（1643 bp），其转录本广泛分布在鸡组织中。饮食治疗不影响十二指肠和盲肠的PCFT mRNA。然而，饲喂5-MTHF日粮的母鸡与基础日粮相比，空肠PCFT mRNA显着降低。 4. PCFT转录本的广泛组织分布表明PCFT在鸡的叶酸转运中起着至关重要的作用。空腹补充5-MTHF可降低空肠PCFT mRNA的表达。这些发现有助于理解禽蛋系统中叶酸运输和PCFT系统的发展。

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