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首页> 外文期刊>Applied Magnetic Resonance >Estimation of the in vivo decay rate of EPR signals for a nitroxide radical in rat brains by a region-selected intensity determination method
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Estimation of the in vivo decay rate of EPR signals for a nitroxide radical in rat brains by a region-selected intensity determination method

机译：通过区域选择强度测定方法估算大鼠脑中一氧化氮自由基的EPR信号的体内衰减率

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摘要

The region-selected intensity determination (RSID) method was proposed to obtain the temporal changes in electron paramagnetic resonance (EPR) signal intensity from a selected region by a stationary magnetic field gradient. To select the region, the subtraction field that was derived from the distance between the center and the projection of the selected region to the direction of the field gradient was applied to the main field. The directions of the stationary magnetic field gradient at a constant strength were systematically changed in a three-dimensional space after each acquisition of the spectrum. All spectra under the field gradient were accumulated and the resultant spectrum was deconvoluted by a spectrum without the field gradient. The center height of the deconvoluted spectrum indicates the signal intensity of the selected region. To verify this method, a phantom or in vivo study was conducted on a 700 MHz radio-frequency EPR spectrometer equipped with a bridged loop-gap resonator. In the temporal EPR measurements of phantoms including a nitroxide radical aqueous solution with and without ascorbic acid, the selected regions were alternatively changed at the position of the two phantoms. The signal intensity derived from the one phantom showed an exponential decay, and for the other phantom, no temporal changes. The spatial resolution of this method was estimated to be 2.7 mm by using a pinpoint phantom that included diphenylpicrylhydrazyl powder. In the in vivo temporal EPR measurements, the selected regions were alternatively changed at the cerebral cortex and the striatum of rats that had received a blood-brain barrier-permeative nitroxide radical. The decay rate of the signal intensity at each region obtained by this method was consistent with those previously reported.
机译：提出了区域选择强度确定（RSID）方法，以通过固定磁场梯度从选定区域获得电子顺磁共振（EPR）信号强度的时间变化。为了选择区域，将从中心和所选区域的投影到场梯度方向的距离得出的减法场应用于主场。每次获取频谱后，在三维空间中系统地更改恒定强度的固定磁场梯度的方向。累积场梯度下的所有光谱，并通过没有场梯度的光谱对卷积的光谱进行反卷积。去卷积频谱的中心高度指示所选区域的信号强度。为了验证该方法，在配有桥式环隙谐振器的700 MHz射频EPR光谱仪上进行了幻像或体内研究。在包括和不带有抗坏血酸的含氮氧化物自由基水溶液的体模的时间EPR测量中，选择的区域在两个体模的位置交替变化。从一个体模得到的信号强度显示出指数衰减，而对于另一个体模，则没有时间变化。该方法的空间分辨率通过使用包含二苯基吡啶甲基肼基粉末的精确模型估计为2.7 mm。在体内时间EPR测量中，已选择的区域在已接受血脑屏障渗透性一氧化氮自由基的大鼠的大脑皮层和纹状体处改变。通过该方法获得的每个区域的信号强度的衰减率与先前报道的一致。

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