Eliminating and inhibiting hydroxylamine oxidation in DEPMPO spin trapping experiments

Liu B; Nie Z; Song YG; Liu YP; Liu KJ; Tian Q; Liu Y

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Eliminating and inhibiting hydroxylamine oxidation in DEPMPO spin trapping experiments

机译：消除和抑制DEPMPO旋转捕集实验中的羟胺氧化

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There exists hydroxylamine impurity in 5-(diethoxyphosphoryl)-5-methyl-1-pyrroline-1-oxide (DEPMPO) despite the purification by column separation and molecular distillation. Slow oxidation of hydroxylamine impurity to nitroxide is caused by a trace amount of transition metal ions in the solution instead by oxygen, and it can be conveniently inhibited upon the addition of diethylenetriaminepentaacetic acid, a transition metal ion chelator. Hydroxylamine impurity in DEPMPO can also be oxidized to electron spin resonance detectable paramagnetic nitroxide in the presence of mild oxidants, such as K3Fe(CN)(6) and CuSO4. The previously recommended procedure for elimination of hydroxylamines was further simplified in the present study. Both hydroxylamine and nitroxide impurities can be removed from the aqueous solution of DEPMPO by activated charcoal treatment.

机译：尽管通过柱分离和分子蒸馏进行了纯化，但是5-（二乙氧基磷酰基）-5-甲基-1-吡咯啉-1-氧化物（DEPMPO）中仍存在羟胺杂质。溶液中的痕量过渡金属离子代替氧气可导致羟胺杂质缓慢氧化为亚硝酸盐，添加二亚乙基三胺五乙酸（一种过渡金属离子螯合剂）可方便地抑制羟胺杂质氧化为亚硝酸盐。在存在轻度氧化剂（例如K3Fe（CN）（6）和CuSO4）的情况下，DEPMPO中的羟胺杂质也可被氧化成电子自旋共振可检测的顺磁性氮氧化物。本研究进一步简化了先前推荐的消除羟胺的程序。可以通过活性炭处理从DEPMPO水溶液中除去羟胺和氮氧化物杂质。

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来源
《Applied Magnetic Resonance》 |2005年第4期|共8页
作者
Liu B; Nie Z; Song YG; Liu YP; Liu KJ; Tian Q; Liu Y;
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正文语种 eng
中图分类电磁学、电动力学;
关键词
HYDROGEN-PEROXIDE; PHOTOSYSTEM-II; FREE-RADICALS; SUPEROXIDE; ADDUCTS; NITRONE; STRESS; ESR; INFLAMMATION; GENETICS;

机译：过氧化氢;光体系II;自由基;超氧化物;添加剂;硝酮;应力;ESR;发炎;遗传;

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专利

1. Eliminating and inhibiting hydroxylamine oxidation in DEPMPO spin trapping experiments [J] . Liu B, Nie Z, Song YG, Applied Magnetic Resonance . 2005,第4期

机译：消除和抑制DEPMPO旋转捕集实验中的羟胺氧化
2. The line asymmetry of electron spin resonance spectra as a tool to determine the cis:trans ratio for spin-trapping adducts of chiral pyrrolines N-oxides: the mechanism of formation of hydroxyl radical adducts of EMPO, DEPMPO, and DIPPMPO in the ische [J] . Culcasi M, Rockenbauer A, Mercier A, Free Radical Biology and Medicine: The Official Journal of the Oxygen Society . 2006,第9期

机译：电子自旋共振谱的线不对称性，用于确定手性吡咯啉N-氧化物自旋俘获加合物的顺式：反式比率：EMPO，DEPMPO和DIPPMPO的羟基自由基加合物形成的机理
3. 5-Diisopropoxyphosphoryl-5-methyl-1-pyrroline N-oxide, DIPPMPO, a crystalline analog of the nitrone DEPMPO: synthesis and spin trapping properties [J] . Florence Chalier and Paul Tordo Journal of the Chemical Society, Perkin Transactions 2 . 2002,第12期

机译：5-二异丙氧基磷酰基-5-甲基-1-吡咯啉N-氧化物，DIPPMPO，硝基DEPMPO的结晶类似物：合成和自旋俘获特性
4. Detection of Oxygen-Centered Radicals Using EPR Spin-Trap DEPMPO: The Effect of Oxygen [C] . MILO? MOJOVI?, MIRJANA VULETI?, GORAN G. BA?I? International Biophysics Symposium . 2005

机译：使用EPR Spin-Trap DEPMPO检测富含氧的自由基：氧气的效果
5. Theory and experiments of electron-hole recombination at silicon/silicon dioxide interface traps and tunneling in thin oxide MOS transistors. [D] . Cai, Jin. 2000

机译：硅/二氧化硅界面陷阱处的电子-空穴复合以及薄氧化物MOS晶体管中的隧穿的理论和实验。
6. Nitrone spin traps and a nitroxide antioxidant inhibit a common pathway of thymocyte apoptosis. [O] . A F Slater, C S Nobel, E Maellaro, 1995

机译：氮自旋阱和一氧化氮抗氧化剂抑制胸腺细胞凋亡的常见途径。
7. Synthesis of 2-Diethoxyphosphoryl-2-Methyl-5-Phenyl-3,4-Dihydro-2H-Pyrrole-1-Oxide (DEPMPO-Ph): a New Radical Spin-Trap [O] . Olive, Gilles, Le Moigne, François, Mercier, Anne, 2001

机译：2-二乙氧基磷酰基-2-甲基-5-苯基-3,4-二氢-2H-吡咯-1-氧化物（DEPMPO-Ph）的合成：新型自由基自旋捕集
8. Study of Fat Oxidation Products with Other Food Components, Antioxidants and Trapping Agents to Inhibit Cross-Linking of Proteins [R] . Andrews, F. A., Thomson, D. A., Underwood, C. E. 1965

机译：脂肪氧化产物与其他食品成分，抗氧化剂和诱捕剂抑制蛋白质交联的研究

Eliminating and inhibiting hydroxylamine oxidation in DEPMPO spin trapping experiments

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