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首页> 外文期刊>Applied Magnetic Resonance >Measurement of microviscosity in vesicular membranes by electron spin resonance
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Measurement of microviscosity in vesicular membranes by electron spin resonance

机译：电子自旋共振法测量水泡膜微粘度

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The seven vesicular formulations CER(-), CER(+), CER(I), PC(-), PC(+), DAG(B) and DAG(I) have been developed using ceramide IIIB (CER), phosphatidylcholine (PC), or diacylglycerides (DAG) as main lipid components. B and I indicate the processed hydrophilic surfactant Brij 58 and Imwitor 375, (+) and (-) symbolize the positive and negative zeta potential. The influence of the bilayer microviscosity on the features of lipid vesicles as dermal drug delivery system is widely discussed, but comparable and reproducible results could not be found. For the measurement of the microviscosity by electron spin resonance the lipophilic spin probe of 14-doxylpalmitoylic acid methyl ester (DPME) was incorporated into the bilayer. Rotational correlation times (tau(c)) between 0.3 and 1.8 ns were calculated. The dynamic viscosities (eta) of eight different medium-chained triglycerides-castor oil mixtures in the range between 25 and 948 mPa center dot s, as well as the corresponding tau(c) values of DPME in these mixtures, were determined to establish a calibration curve for the estimation of the microviscosity. The microviscosities of the vesicle membranes increase from 40 to 565 mPa center dot s in the following order DAG(B) = DAG(I) < PC(-) approximate to CER(I) < CER(-) < CER(+) < PC(+). The microviscosity of 49.8 +/- 2.5 mPa center dot s in pure unsaturated soy PC membranes decreases on adding the sodium cholate to 28.3-29.4 mPa center dot s, whereas the addition of dilauroylphosphatidylcholine in repeated freeze-thaw cycles or 8.5% ethanol in the hydrophilic phase had no influence.
机译：七种囊泡制剂CER（-），CER（+），CER（I），PC（-），PC（+），DAG（B）和DAG（I）已使用神经酰胺IIIB（CER），磷脂酰胆碱（ PC）或二酰基甘油酯（DAG）作为主要脂质成分。 B和I表示已加工的亲水性表面活性剂Brij 58和Imwitor 375，（+）和（-）表示正和负ζ电势。双层微粘度对脂质囊泡作为皮肤药物递送系统的特征的影响已被广泛讨论，但找不到可比较和可重复的结果。为了通过电子自旋共振测量微粘度，将14-羟丙基棕榈酸甲酯（DPME）的亲脂性自旋探针结合到双层中。计算了0.3到1.8 ns之间的旋转相关时间（tau（c））。确定8种不同的中链甘油三酸酯-蓖麻油混合物的动态粘度（η）在25至948 mPa中心点s之间，以及这些混合物中DPME的相应tau（c）值，以建立一个估计微粘度的标准曲线。囊泡膜的微粘度按以下顺序从40 mPa增大到565 mPa中心点：DAG（B）= DAG（I）

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