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首页> 外文期刊>Applied Magnetic Resonance >Pathogen-targeted hydroxyl radical generation during melanization in insect hemolymph: EPR study of a probable cytotoxicity mechanism
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Pathogen-targeted hydroxyl radical generation during melanization in insect hemolymph: EPR study of a probable cytotoxicity mechanism

机译：昆虫血淋巴黑色素化过程中病原体针对的羟基自由基生成：可能的细胞毒性机制的EPR研究

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摘要

The main component of the insect immune system is melanotic encapsulation of pathogenic organisms. Molecular mechanisms of destruction of an encapsulated pathogen are poorly understood. Reactive oxygen species (ROS) are considered as probable cytotoxic agents responsible for destruction of pathogenic organisms in insect hemolymph. In the present work the formation of H2O2 during melanization in Galleria mellonella hemolymph in the presence of catalase inhibitor NaN3 was detected. Enhanced rates of H2O2 generation were observed in the hemolymph of insects activated by injection of bacterial cells. Using spin trapping technique in combination with electron paramagnetic resonance spectroscopy we demonstrated that production of H2O2 in the hemolymph causes the formation of highly toxic reactive oxygen species, hydroxyl radical. However, neither H2O2 nor hydroxyl radical were detected in the absence of NaN3 in agreement with the high catalase activity in the hemolymph. These observations allow us to propose a unique mechanism of pathogen-targeted cytotoxicity based on localized hydroxyl radical generation within a melanotic capsule.
机译：昆虫免疫系统的主要成分是病原生物的黑素化包囊。破坏封装的病原体的分子机制了解甚少。活性氧（ROS）被认为是可能导致昆虫淋巴中病原性生物破坏的细胞毒剂。在目前的工作中，在过氧化氢酶抑制剂NaN3存在下，在梅勒勒商业血淋淋的黑色素化过程中检测到H2O2的形成。在注射细菌细胞而活化的昆虫的血淋巴中观察到过高的H2O2生成速率。使用自旋俘获技术结合电子顺磁共振波谱，我们证明了在血淋巴中产生H2O2会导致形成剧毒的活性氧物种羟基自由基。但是，在没有NaN3的情况下，未检测到H2O2和羟基自由基，这与血淋巴中的高过氧化氢酶活性相一致。这些观察结果使我们能够基于黑色素囊内的局部羟基自由基生成提出病原体靶向细胞毒性的独特机制。

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